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Reprogramming Halomonas for industrial production of chemicals

  • Metabolic Engineering and Synthetic Biology - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Halomonas spp. are able to grow under a high salt concentration at alkali pH, they are able to resist contamination by other microbes. Development of Halomonas spp. as platform production strains for the next-generation industrial biotechnology (NGIB) is intensively studied. Among Halomonas spp., Halomonas bluephagenesis is the best studied one with available engineering tools and methods to reprogram it for production of various polyhydroxyalkanoates, proteins, and chemicals. Due to its contamination resistance, H. bluephagenesis can be grown under open and continuous processes not just in the labs but also in at least 1000 L fermentor scale. It is expected that NGIB based on Halomonas spp. be able to engineer for production of increasing number of products in a competitive manner.

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Abbreviations

ALA:

5-aminolevulinic acid

CDW:

Cell dry weight

CRISPR(i):

Clustered regularly interspaced short palindromic repeats (interference)

GBL:

γ-butyrolactone

IPTG:

Isopropyl β-D-thiogalactoside

NGIB:

Next-generation industrial biotechnology

PHA:

Polyhydroxyalkanoates

PHB:

Poly(3-hydroxybutyrate)

PHBV:

Poly(3-hydroxybutyarte-co-3-hydroxyvalerate)

P3HB4HB:

Poly(3-hydroxybutyarte-co-4-hydroxybutyrate)

RBS:

Ribosomal-binding site

RNAP:

RNA polymerase

SEVA:

Standard European Vector Architecture

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Acknowledgements

This study has been supported by National Natural Science Foundation of China (Grant No. 21761132013 and 31430003).

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Author notes

  1. Xiangbin Chen, Linping Yu, and Guanqing Qiao have contributed equally to this work.

Authors and Affiliations

  1. MOE Lab of Bioinformatics, School of Life Sciences, Tsinghua University, 100084, Beijing, China

    Xiangbin Chen, Linping Yu, Guanqing Qiao & Guo-Qiang Chen

  2. Center for Synthetic and Systems Biology, Tsinghua University, 100084, Beijing, China

    Xiangbin Chen, Linping Yu, Guanqing Qiao & Guo-Qiang Chen

  3. Tsinghua-Peking Center for Life Sciences, Tsinghua University, 100084, Beijing, China

    Xiangbin Chen, Linping Yu, Guanqing Qiao & Guo-Qiang Chen

  4. Manchester Institute of Biotechnology, Centre for Synthetic Biology, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK

    Guo-Qiang Chen

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  1. Xiangbin Chen
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  2. Linping Yu
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  3. Guanqing Qiao
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Correspondence to Guo-Qiang Chen.

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Submitted to “Journal of Industrial Microbiology and Biotechnology” (JIMB) for the Special Issue on “Synthetic Biology for the Biotechnology Industry”.

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Chen, X., Yu, L., Qiao, G. et al. Reprogramming Halomonas for industrial production of chemicals. J Ind Microbiol Biotechnol 45, 545–554 (2018). https://doi.org/10.1007/s10295-018-2055-z

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