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Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic Bacillus coagulans WCP10-4

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Abstract

Dry yeast cells (DYC) were used as a cheap nitrogen source to replace expensive yeast extract (YE) for L-lactic acid production by thermophilic Bacillus coagulans. Cassava starch (200 g·L–1) was converted to L-lactic acid by simultaneous saccharification and fermentation using Bacillus coagulans WCP10-4 at 50 °C in the presence of 20 g·L–1 of DYC, giving 148.1 g·L–1 of Llactic acid at 27 h with a productivity of 5.5 g·L–1·h–1 and a yield of 92%. In contrast, 154.4 g·L–1 of lactic acid was produced at 24 h with a productivity of 6.4 g·L–1·h–1 and a yield of 96% when equal amount of YE was used under the same conditions. Use of pre-autolyzed DYC at 50 °C for overnight slightly improved the lactic acid titer (154.5 g·L–1) and productivity (7.7 g·L–1·h–1) but gave the same yield (96%).

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Authors and Affiliations

  1. Institute of Chemical and Engineering Sciences, A*STAR, 1 Pesek Road, Jurong Island, 627833, Singapore

    Kim Yng Ooi & Jin Chuan Wu

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  1. Kim Yng Ooi
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  2. Jin Chuan Wu
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Correspondence to Jin Chuan Wu.

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Dedicated to the 120th Anniversary of Tianjin University

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Ooi, K.Y., Wu, J.C. Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic Bacillus coagulans WCP10-4. Front. Chem. Sci. Eng. 9, 381–385 (2015). https://doi.org/10.1007/s11705-015-1534-2

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  • DOI: https://doi.org/10.1007/s11705-015-1534-2

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