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Attenuation of hydrogen peroxide-induced oxidative stress in SH-SY5Y cells by three flavonoids from Acer okamotoanum

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Abstract

The flavonoids quercitrin, isoquercitrin, and afzelin were isolated from the ethyl acetate fraction of Acer okamotoanum. The effectiveness of these flavonoids in protecting human cells was investigated using SH-SY5Y neuronal cells. Oxidative stress was induced by hydrogen peroxide (H2O2) and cells treated with flavonoids had an increased viability as compared with untreated cells. The flavonoid-treated cells showed significantly less reactive oxygen species production and lactase dehydrogenase release than the untreated cells. Especially, quercitrin and isoquercitrin showed the strongest protective effects against oxidative stress among several tested flavonoids as determined by the results for cell viability, reactive oxygen species (ROS) production, and lactate dehydrogenase release. Furthermore, we measured the expression of inflammation- and apoptosis-related proteins. These flavonoids attenuated inflammation by downregulating the expression of cyclooxygenase-2, inducible nitric oxide synthase, and apoptotic signaling via inhibiting caspase activation. The present results suggest that three flavonoids of A. okamotoanum protect against cellular oxidative stress possibly through regulating inflammation, apoptosis, and ROS-scavenging.

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Acknowledgements

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A01058868), Republic of Korea. This research was supported by Global PH.D Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016_H1A2A1906940).

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Authors and Affiliations

  1. Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University, Busan, 46241, Republic of Korea

    Ji Hyun Kim & Eun Ju Cho

  2. Department of Integrative Plant Science, Chung-Ang University, Anseong, 17546, Republic of Korea

    Norman G. Quilantang & Sanghyun Lee

  3. Department of Food Science, Gyeongnam National University of Science and Technology, Jinju, 52725, Republic of Korea

    Hyun Young Kim

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  1. Ji Hyun Kim
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  2. Norman G. Quilantang
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Correspondence to Sanghyun Lee or Eun Ju Cho.

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Kim, J.H., Quilantang, N.G., Kim, H.Y. et al. Attenuation of hydrogen peroxide-induced oxidative stress in SH-SY5Y cells by three flavonoids from Acer okamotoanum. Chem. Pap. 73, 1135–1144 (2019). https://doi.org/10.1007/s11696-018-0664-7

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