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Development of an ic-ELISA and a TRFICA for the detection of fipronil with a new hapten design

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Abstract

Based on the new hapten of fipronil, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and time-resolved fluorescent immunochromatographic assay (TRFICA) for the determination of fipronil were developed. The sensitivity (IC50) of the ic-ELISA method was 2.33 ± 0.14 ng mL−1 (n = 3), and the limit of detection (LOD) (IC20) was 0.27 ± 0.02 ng mL−1 (n = 3). The cross-reaction rates of the three fipronil metabolites were all higher than 66.7%, and those of the three analogs were no more than 0.05%. The TRFICA was optimized with a handheld reader. The LOD was 0.862 ng mL−1 (n = 3). In this study, the pretreatment methods of samples such as Chinese cabbage, spinach, apple and egg, were explored. Methanol and acetonitrile were used to extract different food samples and the diluents were optimized. Three concentration levels in the spiked food samples were detected by the two methods. The range of recovery was 88.65–116.32% for ic-ELISA and 95.48–112.61% for TRFICA. In this study, the accuracy of the two methods was also judged by testing commercial quality control samples. The results showed that the accuracy was 83.75%–113.33% for ic-ELISA and 98.33–107.13% for TRFICA.

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Acknowledgements

This work was supported by The Project program of Tianjin Key Laboratory of Food Quality and Health, Tianjin University of Science and Technology, P. R. China (No. TJS202104) and the National Program on Key Research Project of China (No. 2018YFE0200402)

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Authors and Affiliations

  1. Tianjin Key Laboratory of Food Quality and Health, State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China

    Junwei Liu, Rui Zhang & Yang Lu

  2. TianJin International Joint Academy of Biomedicine, Tianjin, 300457, China

    Junwei Liu, Yuqing Wu, Chenchen Liu & Yan Cai

  3. College of Pharmacy, Nankai University, Tianjin, 300350, China

    Yuqing Wu & Chenchen Liu

Authors
  1. Junwei Liu
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  2. Yuqing Wu
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  3. Chenchen Liu
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  4. Yan Cai
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  5. Rui Zhang
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  6. Yang Lu
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Contributions

Junwei Liu: Conceptualization, methodology, project administration, writing—review and editing, resources; Yuqing Wu: Investigation, data curation, formal analysis, validation, writing—original draft, visualization; Chenchen Liu: Investigation, validation, formal analysis, data curation, writing—original draft; Yan Cai: Methodology, validation, formal analysis; Yang Lu: Conceptualization, methodology, supervision, funding acquisition, writing—review and editing.

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Correspondence to Junwei Liu or Yang Lu.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethics Committee of Tianjin University of Science and Technology for the use of Laboratory Animals.

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Liu, J., Wu, Y., Liu, C. et al. Development of an ic-ELISA and a TRFICA for the detection of fipronil with a new hapten design. Food Measure 18, 2561–2572 (2024). https://doi.org/10.1007/s11694-023-02336-2

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