Abstract
The purpose of this study was to evaluate the antioxidant properties and total phenolic content of a dairy product (yogurt) supplemented with Thymus willdenowii (synonym: Thymus hirtus) essential oil. The evaluation of the antioxidant and antibacterial activities of the oil against food spoilage bacteria and lactic acid strains was also assessed. The chemical composition of the oil was obtained by GC/MS. The evaluation of the antioxidant activity of a fortified yogurt with this oil was assessed by Oxygen radical absorbance capacity test (ORAC). The main component of the oil was Linalool (89.55%). DPPH, and Ferric reducing power revealed a weak antioxidant activity (IC50 > 1600 µg/mL, > 800 µg/mL respectively) while a moderate activity was obtained with ABTS and CUPRAC tests (38.06 ± 0.30, 56.15 ± 1.07 µg/mL respectively). A high concentration of the oil is needed to delay the inhibition of the β-carotene (> 800 µg/mL). Thus, a strong antibacterial activity was observed against the pathogenic strains with the high inhibition diameter for B.cereus ATCC10876 (31.75 ± 1.50 mm) while a weak effect was observed against lactic acid bacteria. A significant decrease in the antioxidant potential of non-supplemented yogurt was observed from day 1 to 21 while there was no significant difference in ORAC of supplemented yogurt with the oil. The maximum of total phenolic content was observed in day 21 of cold storage (252.92 ± 3.01 µg GAE/mL of yogurt). The essential oil studied could be an effective natural additive to yogurt avoiding the use of synthetic preservatives.
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A. Hassoun, M. Carpena, M.A. Prieto, J. Simal-Gandara, F. Özogul, Y. Özogul, ÖE. Çoban, M. Guðjónsdóttir, F.J. Barba, F.J. Marti-Quijal, A.R. Jambrak, N. Maltar-Strmečki, J.G. Kljusurić, J.M. Regenstein, Antioxidants 9, 882 (2020). https://doi.org/10.3390/antiox9090882
K. Wińska, W. Mączka, J. Łyczko, M. Grabarczyk, A. Czubaszek, A. Szumny, Molecules 24, 2130 (2019). https://doi.org/10.3390/molecules24112130
D.N. Reddy, Essential Oils Extracted from Medicinal Plants and Their Applications, in Natural Bio-active Compounds, ed. by M. Akhtar, M. Swamy, U. Sinniah (Springer, Singapore, 2019), https://doi.org/10.1007/978-981-13-7154-7_9
M. Kačániová, L. Galovičová, E. Ivanišová, N.L. Vukovic, J. Štefániková, V. Valková, P. Borotová, J. Žiarovská, M. Terentjeva, S. Felšöciová, E. Tvrdá, Foods 9, 282 (2020). https://doi.org/10.3390/foods9030282
S. Prabuseenivasan, M. Jayakumar, S. Ignacimuthu, BMC Complement. Altern. Med. 6, 39 (2006). https://doi.org/10.1186/1472-6882-6-39
M. Catauro, F. Bollino, E. Tranquillo, L. Sapio, M. Illiano, I. Caiafa, S. Naviglio, J. Essent. Oil Res. 29, 461 (2017). https://doi.org/10.1080/10412905.2017.1351405
K. Böhme, J. Barros-Velázquez, P. Calo-Mata, S.P. Aubourg, in Antimicrobial Compounds, edited by T. G. Villa and P. Veiga-Crespo (Springer Berlin Heidelberg, Berlin, Heidelberg, 2014), pp. 51–81.https://doi.org/10.1007/978-3-642-40444-3_3
A.P. Mishra, H.P. Devkota, M. Nigam, C.O. Adetunji, N. Srivastava, S. Saklani, I. Shukla, L. Azmi, M.A. Shariati, H.D. Melo, Coutinho, A. Mousavi Khaneghah, LWT 133, 110116 (2020). https://doi.org/10.1016/j.lwt.2020.110116
N.R. Menković, K.P. Šavikin, G.M. Zdunić, G. Gojgić-Cvijović, J. Essent. Oil Res. 21, 89 (2009). https://doi.org/10.1080/10412905.2009.9700119
S. Roller, N. Ernest, J. Buckle, J. Altern. Complement. Med. 15, 275 (2009). https://doi.org/10.1089/acm.2008.0268
F. Yangilar, P.O. Yildiz, J. Food Process. Preserv. 42, e13332 (2018). https://doi.org/10.1111/jfpp.13332
H. Punia, J. Tokas, A. Malik, S. Sangwan, S. Baloda, N. Singh, S. Singh, A. Bhuker, P. Singh, S. Yashveer, S. Agarwal, V.S. Mor, Molecules 25, 3328 (2020). https://doi.org/10.3390/molecules25153328
I.T. Khan, M. Nadeem, M. Imran, R. Ullah, M. Ajmal, M.H. Jaspal, Lipids Health Dis 18, 41 (2019). https://doi.org/10.1186/s12944-019-0969-8
A. Sharifi-Rad, G. Sureda, M. Tenore, M. Daglia, M. Sharifi-Rad, R. Valussi, M. Tundis, M. Sharifi-Rad, A. Loizzo, R. Ademiluyi, S. Sharifi-Rad, Ayatollahi, M. Iriti, Molecules 22, 70 (2017). https://doi.org/10.3390/molecules22010070
A. Zeghib, C.-A. Calliste, A. Simon, R. Charfeddine, M. Aouni, J.-L. Duroux, A. Kabouche, Z. Kabouche, Nat. Prod. Res. 35, 845 (2021). https://doi.org/10.1080/14786419.2019.1602830
M. Ouknin, A. Romane, J. Costa, L. Majidi, Nat. Prod. Res. 33, 2398 (2019). https://doi.org/10.1080/14786419.2018.1443089
D. Sarfaraz, M. Rahimmalek, G. Saeidi, Sci. Rep. 11, 5019 (2021). https://doi.org/10.1038/s41598-021-84449-6
G. Vardar-Ünlü, F. Candan, A. Sökmen, D. Daferera, M. Polissiou, M. Sökmen, E. Dönmez, B. Tepe, J. Agric. Food Chem. 51, 63 (2003). https://doi.org/10.1111/jfpp.13332
M.C. Rota, A. Herrera, R.M. Martínez, J.A. Sotomayor, M.J. Jordán, Food Control 19, 681 (2008). https://doi.org/10.1016/j.foodcont.2007.07.007
CFR - Code of FederaL ReguLations TitLe 21. https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?CFRPart=182&showFR=1
D.J. Daferera, B.N. Ziogas, M.G. Polissiou, J. Agric. Food Chem. 48, 2576 (2000). https://doi.org/10.1021/jf990835x
J. Safaei-Ghomi, A.H. Ebrahimabadi, Z. Djafari-Bidgoli, H. Batooli, Food Chem. 115, 1524 (2009). https://doi.org/10.1016/j.foodchem.2009.01.051
M.S. Blois, Nature 181, 1199 (1958). https://doi.org/10.1038/1811199a0
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Free Radic. Biol. Med. 26, 1231 (1999). https://doi.org/10.1016/S0891-5849(98)00315-3
R. Apak, K. Güçlü, M. Özyürek, S.E. Karademir, J. Agric. Food Chem. 52, 7970 (2004). https://doi.org/10.1021/jf048741x
M. Oyaizu, Jpn J. Nutr. Diet. 44, 307 (1986). https://doi.org/10.5264/eiyogakuzashi.44.307
G.J. Marco, J. Am. Oil Chem. Soc. 45, 594 (1968). https://doi.org/10.1007/BF02668958
A. Chebaibi, Z. Marouf, F. Rhazi-Filali, M. Fahim, A. Ed-Dra, Phytothérapie 14, 355 (2016). https://doi.org/10.1007/s10298-015-0996-1
M. Michael, R.K. Phebus, K.A. Schmidt, Int. Dairy J. 20, 665 (2010)
A. Perna, I. Intaglietta, A. Simonetti, E. Gambacorta, J. Dairy Sci. 97, 6662 (2014). https://doi.org/10.3168/jds.2013-784
V.L. Singleton, J.A. Rossi, Am. J. Enol. Vitic 16, 144 (1965)
L. Müller, S. Gnoyke, A.M. Popken, V. Böhm, LWT - Food Science and Technology 43, 992 (2010). https://doi.org/10.1016/j.lwt.2010.02.004
M.E. Alañón, L. Castro-Vázquez, M.C. Díaz-Maroto, M.H. Gordon, M.S. Pérez-Coello, Food Chem. 128, 997 (2011). https://doi.org/10.1016/j.foodchem.2011.04.005
N. Bernaert, D. De Paepe, C. Bouten, H. De Clercq, D. Stewart, E. Van Bockstaele, M. De Loose, B. Van Droogenbroeck, Food Chem. 134, 669 (2012). https://doi.org/10.1016/j.foodchem.2012.02.159
Y. Rao. Fu, Y. Zhang. Guo, F. Chen, South Afr. J. Bot. 93, 98 (2014). https://doi.org/10.1016/j.sajb.2014.04.003
E.B. Bnina, S. Hammami, M. Daami-Remadi, I. Cheraif, H. Hajjlaoui, H.B. Jannet, M.B. Said, Z. Mighri, J. Essent. Oil Res. 21, 567 (2009). https://doi.org/10.1080/10412905.2009.9700247
M.G. Miguel, C. Gago, M.D. Antunes, C. Megías, I. Cortés-Giraldo, J. Vioque, A.S. Lima, A.C. Figueiredo, Evidence-Based Complementary and Alternative Medicine 2015, 1 (2015).https://doi.org/10.1155/2015/851721
F. Radi, M. Bouhrim, H. Mechchate, M. Al-zahrani, A. Qurtam, A. Aleissa, D. Aziz, N. Hadaq, Z. Touria, Plants 11, 1 (2021). https://doi.org/10.3390/plants11010015
F. Guesmi, M. Ben Ferhat, M. Mejri, A. Landoulsi, Lipids Health Dis 13, 114 (2014). https://doi.org/10.1186/1476-511X-13-114
F. Guesmi, S. Issam, H. Najla, A. Landoulsi, Annual Res. Rev. Biology 1 (2018).https://doi.org/10.9734/ARRB/2018/43583
T. Baj, I. Korona-Głowniak, R. Kowalski, A. Malm, Open Chem. 16, 317 (2018). https://doi.org/10.1515/chem-2018-0032
S. Djebili, M. Taş, A. Bouguerra, S. Küçükaydın, C. Ozgur, M. Duru, M. Barkat, J. Food Meas. Charact. 16, 987 (2021). https://doi.org/10.1515/chem-2018-003210.1007/s11694-021-01220-1
F. Guesmi, I. Saidi, H. Bouzenna, N. Hfaiedh, A. Landoulsi, South Afr. J. Bot. 127, 234 (2019). https://doi.org/10.1016/j.sajb.2019.09.004
I. Bakirci, Nahrung 43, 333 (1999).https://doi.org/10.1002/(SICI)1521-3803(19991001)43:5<333::AID-FOOD333>3.0.CO;2-T
A.C. Guimarães, L.M. Meireles, M.F. Lemos, M.C.C. Guimarães, D.C. Endringer, M. Fronza, R. Scherer, Molecules 24, 2471 (2019). https://doi.org/10.3390/molecules24132471
L.D. Gann, A.F.T. Council, C.R. Hodges, Antimicrobial Activity of Essential Oils and Their Components Against Lactic Acid Bacteria (2013).https://trace.tennessee.edu/utk_gradthes/2604
H. Zengin, A. Baysal, Molecules 19, 17773 (2014). https://doi.org/10.3390/molecules1911177733
H. Nikaido, Science 264, 382 (1994). https://doi.org/10.1126/science.8153625
A. Emad. Shalaby, S.M.M. Shanab, NISCAIR-CSIR, India 556 (2013).https://www.researchgate.net/publication/287536387
H. Rodríguez, J.A. Curiel, J.M. Landete, B. de las Rivas, F.L. de Felipe, C. Gómez-Cordovés, J.M. Mancheño, R. Muñoz, Int. J. Food Microbiol. 132, 79 (2009). https://doi.org/10.1016/j.ijfoodmicro.2009.03.025
T. Gjorgievski, M. Dimitrovska, and Shariati (2014), Journal of Hygienic Engineering and Design 615 (2014).https://www.researchgate.net/publication/286440609_DETERMINATION_OF_THE_ANTIOXIDANT_ACTIVITY_IN_YOGURT
A.B. Shori, J. Taibah Univ. Sci. 14, 1000 (2020). https://doi.org/10.1080/16583655.2020.1798072
A. Fardet, E. Rock, Nutr. Res. Rev. 31, 52 (2018). https://doi.org/10.1017/S0954422417000191
A. Citta, A. Folda, V. Scalcon, G. Scutari, A. Bindoli, M. Bellamio, E. Feller, M.P. Rigobello, Food Sci. Nutr. 5, 1079 (2017). https://doi.org/10.1002/fsn3.493
W. Liao, W. Badri, A.H. Alhibshi, E. Dumas, S. Ghnimi, A. Gharsallaoui, A. Errachid, A. Elaissari, in Black Cumin (Nigella Sativa) Seeds: Chemistry, Technology, Functionality, and Applications, edited by M. Fawzy Ramadan (Springer International Publishing, Cham, 2021), pp. 433–455.http://link.springer.com/https://doi.org/10.1007/978-3-030-48798-0_28
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Benguedouar, K., Betina, S.B., Erenler, R. et al. Evaluation of the antioxidant properties and total phenolic content of a dairy product (yogurt) supplemented with Thymus willdenowii essential oil from Algeria. Food Measure 16, 3568–3577 (2022). https://doi.org/10.1007/s11694-022-01455-6
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DOI: https://doi.org/10.1007/s11694-022-01455-6