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Effect of Co-60 gamma irradiation on Aspergillus flavus, Aflatoxin B1 and qualitative characteristics of pistachio nuts (Pistacia vera L.)

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Abstract

In this study the effect of different doses of gamma irradiation (0, 0.5, 1, 1.5, 2, 4 and 6 kGy) on viable spore population of Aspergillus flavus, Aflatoxin B1 (AFB1) concentration and qualitative characteristics of pistachio samples were investigated. The results indicated that gamma irradiation at doses of 4 and 6 kGy reduced the viable spore population of A. flavus about 5 log. The maximum degradation of AFB1 in pistachio samples was 73.26% and 83.36% that was observed at doses of 4 and 6 kGy, respectively. The total phenol content (TPC) increased at dose of 2 kGy, but it was reduced at higher doses of irradiation. The increase of irradiation doses up to 4 kGy significantly increased the antioxidant activity, while irradiation dose of 6 kGy led to a reduction in antioxidant activity. All irradiation doses increased the amount of malondialdehyde in pistachio samples and the highest amount of malondialdehyde was observed at dose of 6 kGy. The chlorophyll and carotenoid content was decreased at all absorbed doses of gamma irradiation and affected the color features and resulted to a darker color of pistachio samples. Gamma irradiation slightly decreased the solubility of proteins and altered the pattern and intensity of the protein bands. The obtained results showed that gamma irradiation at doses higher than 2 kGy is capable of controlling and reducing the contamination of pistachio samples, while lower doses (kGy ≤ 2) of gamma irradiation caused minimum changes in pistachio samples quality.

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References

  1. S.F. Mexis, M.G. Kontominas, Effect of gamma irradiation on the physico-chemical and sensory properties of raw shelled peanuts (Arachis hypogaea L.) and pistachio nuts (Pistacia vera L.). J. Sci. Food Agric. 89, 867–875 (2009)

    Article  CAS  Google Scholar 

  2. M. Akbari, M. Farajpour, M. Aalifar, M. Sadat Hosseini, Gamma irradiation affects the total phenol, anthocyanin and antioxidant properties in three different Persian pistachio nuts. Nat. Prod. Res. 32, 322–326 (2018)

    Article  CAS  Google Scholar 

  3. P. Sánchez-Bel, I. Egea, F. Romojaro, M.C. Martínez-Madrid, Sensorial and chemical quality of electron beam irradiated almonds (Prunus amygdalus). LWT-Food Sci. Technol. 41, 442–449 (2008)

    Article  Google Scholar 

  4. S.Z. Iqbal, I.A. Bhatti, M.R. Asi, M. Zuber, M. Shahid, I. Parveen, Effect of γ irradiation on fungal load and aflatoxins reduction in red chillies. Radiat. Phys. Chem. 82, 80–84 (2013)

    Article  CAS  Google Scholar 

  5. I. Ghanem, M. Orfi, M. Shamma, Effect of gamma radiation on the inactivation of aflatoxin B1 in food and feed crops. Braz. J. Microbiol. 39, 787–791 (2008)

    Article  CAS  Google Scholar 

  6. European Commission, Commission regulation no 165/2010 of 26 February 2010 on amending regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards aflatoxins. Official Journal of the European Union, L50, 8–12, 27.2 (2010)

  7. U. Gecgel, T. Gumus, M. Tasan, O. Daglioglu, M. Arici, Determination of fatty acid composition of γ-irradiated hazelnuts, walnuts, almonds, and pistachios. Radiat. Phys. Chem. 80, 578–581 (2011)

    Article  CAS  Google Scholar 

  8. T. Calado, A. Venâncio, L. Abrunhosa, Irradiation for mold and mycotoxin control: a review. Compr. Rev. Food Sci. Food Saf. 13, 1049–1061 (2014)

    Article  CAS  Google Scholar 

  9. S.K. Güler, S.Z. Bostan, A.H. Çon, Effects of gamma irradiation on chemical and sensory characteristics of natural hazelnut kernels. Post Biol. Technol. 123, 12–21 (2017)

    Article  Google Scholar 

  10. S. Aquino, F. Ferreira, D.H.B. Ribeiro, B. Corrêa, R. Greiner, A.L.C.H. Villavicencio, Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize. Braz. J. Microbiol. 36, 352–356 (2005)

    Article  Google Scholar 

  11. Y. Sen, B. Onal-Ulusoy, M. Mutlu, Detoxification of hazelnuts by different cold plasmas and gamma irradiation treatments. Innov. Food Sci. Emerg. Technol. 54, 252–259 (2019)

    Article  CAS  Google Scholar 

  12. A. Gorran, M. Farzaneh, M. Shivazad, M. Rezaeian, A. Ghassempour, Aflatoxin B1-reduction of Aspergillus flavus by three medicinal plants (Lamiaceae). Food Control 31, 218–223 (2013)

    Article  CAS  Google Scholar 

  13. S. Khodavaisy, A. Maleki, B. Hossainzade, S. Rezai, F. Ahmadi, A. Validi, Occurrence of fungal contamination in pistachio and peanut samples from retail shops in Sanandaj province, Iran. Afr. J. Microbiol. Res. 6, 6781–6784 (2012)

    Article  Google Scholar 

  14. M. Hojjati, L. Noguera-Artiaga, A. Wojdyło, Á.A. Carbonell-Barrachina, Effects of microwave roasting on physicochemical properties of pistachios (Pistaciavera L.). Food Sci. Biotechnol. 24, 1995–2001 (2015)

    Article  CAS  Google Scholar 

  15. A. Papastergiadis, E. Mubiru, H. Van Langenhove, B. De Meulenaer, Malondialdehyde measurement in oxidized foods: evaluation of the spectrophotometric thiobarbituric acid reactive substances (TBARS) test in various foods. J. Agric Food Chem. 60, 9589–9594 (2012)

    Article  CAS  Google Scholar 

  16. M. Alinezhad, M. Hojjati, H. Barzegar, S. Shahbazi, H. Askari, Effect of gamma irradiation on the physicochemical properties of pistachio (Pistacia vera L.) nuts. J. Food Meas. Character. 15, 199–209 (2021)

    Article  Google Scholar 

  17. M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)

    Article  CAS  Google Scholar 

  18. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970)

    Article  CAS  Google Scholar 

  19. T. Boonchoo, P. Jitareerat, S. Photchanachai, A. Chinaphuti, Effect of gamma irradiation on Aspergillus flavus and brown rice quality during storage. Proc 2nd Int Symp on the New Frontier of Irradiated food and non food products, KMUTT, Bangkok, Thailand (2005)

  20. J. Farkas, Irradiation for better foods. Trends Food Sci. Technol. 17, 148–152 (2006)

    Article  CAS  Google Scholar 

  21. M. Jalili, S. Jinap, M. Noranizan, Aflatoxins and ochratoxin a reduction in black and white pepper by gamma radiation. Radiat. Phys. Chem. 8, 1786–1788 (2012)

    Article  Google Scholar 

  22. A.K. Dixit, D. Bhatnagar, V. Kumar, A. Rani, J. Manjaya, D. Bhatnagar, Gamma irradiation induced enhancement in isoflavones, total phenol, anthocyanin and antioxidant properties of varying seed coat colored soybean. J. Agric. Food Chem. 58, 4298–4302 (2010)

    Article  CAS  Google Scholar 

  23. K.H. Kim, M.S. Kim, H.G. Kim, H.S. Yook, Inactivation of contaminated fungi and antioxidant effects of peach (Prunus persica L. Batsch cv Dangeumdo) by 0.5–2 kGy gamma irradiation. Radiat. Phys. Chem. 79, 495–501 (2010)

    Article  CAS  Google Scholar 

  24. P. Sirisoontaralak, A. Noomhorm, Changes to physicochemical properties and aroma of irradiated rice. J. Stored Prod. Res. 42, 264–276 (2006)

    Article  CAS  Google Scholar 

  25. E. Gölge, G. Ova, The effects of food irradiation on quality of pine nut kernels. Radiat. Phys. Chem. 77, 365–369 (2008)

    Article  Google Scholar 

  26. M. Ramamurthy, A. Kamat, A. Kakatkar, N. Ghadge, B. Bhushan, M. Alur, Improvement of shelf-life and microbiological quality of minimally processed refrigerated capsicum by gamma irradiation. Int. J. Food Sci. Nutr. 55, 291–299 (2004)

    Article  CAS  Google Scholar 

  27. M.W. Byun, C. Jo, K.H. Lee, K.S. Kim, Chlorophyll breakdown by gamma irradiation in a model system containing linoleic acid. J. Am. Oil Chem. Soc. 79, 145–150 (2002)

    Article  CAS  Google Scholar 

  28. A.L.P. Kiong, A.G. Lai, S. Hussein, A.R. Harun, Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation. Am. Eurasian J. Sustain. Agric. 2, 135–149 (2008)

    Google Scholar 

  29. A.E. Afify, M.M. Rashed, E.A. Mahmoud, H.S. El-Beltagi, Effect of gamma radiation on protein profile, protein fraction and solubility’s of three oil seeds: soybean, peanut and sesame. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39, 90–98 (2011)

    Article  CAS  Google Scholar 

  30. R. Noorbakhsh, S.A. Mortazavi, M. Sankian, F. Shahidi, S.J. Maleki, L.R. Nasiraii, Influence of processing on the allergenic properties of pistachio nut assessed in vitro. J. Agric. Food Chem. 58, 10231–10235 (2010)

    Article  CAS  Google Scholar 

  31. V. Krishnan, A. Singh, V. Thimmegowda, B. Singh, A. Dahuja, R.D. Rai, Low gamma irradiation effects on protein profile, solubility, oxidation, scavenger ability and bioavailability of essential minerals in black and yellow Indian soybean (Glycine max L.) varieties. J. Radioanal. Nucl. Chem. 307, 49–57 (2016)

    Article  CAS  Google Scholar 

  32. V.Y. Naei, M. Sankian, M. Moghadam, N. Farshidi, S.H. Ayati, F. Hamid, The influence of gamma radiation processing on the allergenicity of main pistachio allergens. Rep. Biochem. Mol. Biol. 7, 150 (2019)

    Google Scholar 

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Acknowledgements

We are grateful to the respected authorities of Agricultural Sciences and Natural Resources University of Khuzestan for supporting this project.

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Authors and Affiliations

  1. Department of Food Science and Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran

    Mina Makari & Mohammad Hojjati

  2. Nuclear Agriculture School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj, Iran

    Samira Shahbazi & Hamed Askari

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  1. Mina Makari
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Correspondence to Mohammad Hojjati.

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Makari, M., Hojjati, M., Shahbazi, S. et al. Effect of Co-60 gamma irradiation on Aspergillus flavus, Aflatoxin B1 and qualitative characteristics of pistachio nuts (Pistacia vera L.). Food Measure 15, 5256–5265 (2021). https://doi.org/10.1007/s11694-021-01060-z

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