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Protective Effect of Some Essential Oils Against Gamma-Radiation Damages in Tetrahymena pyriformis Exposed to Cobalt-60 Source

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Abstract

The main purpose of this study was to investigate the protective effect of Rosmarinus officinalis, Origanum compactum, Lavandula angustifolia, and Eucalyptus globulus essential oils (EOs) against gamma-radiation-induced damages on Tetrahymena pyriformis growing in presence of cobalt-60 source. The chemical composition of the 4 EOs was analyzed by gas chromatography–mass spectrometry. The protective effects of EOs on growth, on morphology, and on some metabolic enzymes and antioxidant markers have been evaluated. Thus, addition of EOs significantly improves the growth parameters (generation number and time) in irradiating conditions. All EOs allowed restoring growth parameters over more than 90% compared to the controls. The morphological analysis indicated that T. pyriformis cells growing in irradiating conditions were able to regain their normal form in presence of the different EOs. Our results indicate that the 4 EOs also have protective effects on some metabolic enzymes. They allowed recovering totally or partially the glyceraldehyde 3-phosphate dehydrogenase and the succinate dehydrogenase activities compared to the controls. Moreover, the addition of EOs reduced the lipid peroxidation level and decreased the activities of catalase and superoxide dismutase induced by the gamma-radiation exposure. A more pronounced protective effect was found for O. compactum and L. angustifolia EOs compared to R. officinalis and E. globulus EOs. These results suggest that the studied EOs are efficient natural antioxidants that could offer protection against gamma-radiation-induced damages and can therefore be useful in clinical medicine.

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Acknowledgements

The authors would like to thank Dr. Moussaif A, Dr. Sbihi Y, Dr. Bouayyadi A, Mr Amaarof M, Mr Moulahid H and Mrs Bellemjid N (CNESTEN, Rabat, Morocco) for their assistance and helpful suggestions.

Funding

This work was supported by the National Center for Nuclear Energy, Science and Technology (CNESTEN) as an internal project grant.

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Authors and Affiliations

  1. Biotechnology and Biomolecule Engineering Unit, National Center for Nuclear Energy, Science and Technology (CNESTEN), BP 1382, 10001, Rabat, Morocco

    Soukaina Ziyadi, Abdelghani Iddar, Mohammed El Mzibri & Adnane Moutaouakkil

  2. Health and Environment Laboratory, Faculty of Sciences Aïn-Chock, Hassan II University, Km 8 Route d’El Jadida, Mâarif, BP 5366, 20100, Casablanca, Morocco

    Soukaina Ziyadi, Khadija Ridaoui & Mostafa Kabine

  3. Faculty of Medicine, Mohammed VI University of Health Sciences (UM6SS), Bld Mohammed Taïeb Naciri, 82403, Casablanca, Morocco

    Nadia Errafiy

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  1. Soukaina Ziyadi
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Contributions

Conceptualization: [AI, AM]; Methodology: [SZ, AI, NE, AM]; Formal analysis and investigation: [SZ, NE, KR, MK, AM]; Writing-original draft preparation: [AI, AM]; Writing-review and editing: [AI, AM]; Funding acquisition: [ME]; Supervision: [ME, AM]. All authors read and approved the final version of the manuscript.

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Correspondence to Adnane Moutaouakkil.

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Ziyadi, S., Iddar, A., Errafiy, N. et al. Protective Effect of Some Essential Oils Against Gamma-Radiation Damages in Tetrahymena pyriformis Exposed to Cobalt-60 Source. Curr Microbiol 79, 279 (2022). https://doi.org/10.1007/s00284-022-02924-3

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