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Development and application of a novel immunoaffinity test column assay for visual detection of cloxacillin residues in animal-derived food samples

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Abstract

Nowadays, the abuse of cloxacillin (CLOX) in livestock has generated considerable problems for food safety. In this study, a rapid and visual immunoaffinity test column (IATC) assay was developed for the detection of CLOX residues in animal derived food. The novel IATC assay was used by traditional immune competition and gel separation methods. Traditional immune competition could detect CLOX, and gel separation could reduce the effect of matrix in the samples. The established IATC assay was successfully applied to detect CLOX residue in food samples, with a visual limit of detection of 25 ng/g. The results obtained with the proposed IATC assay agreed well with those obtained by liquid chromatography–tandem mass spectrometry. It indicated that the developed method could be an effective analytical tool to monitor CLOX residues in food products.

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Acknowledgements

This work was supported by China Hebei Provincial Science and Technology Project (Grant No. QN2016242), Hebei science and technology Project (Grant No. 16236802D-5), Shijiazhuang Science and Technology Project (Grant Nos. 181170054A, 18T000022A, 191170082A, 191170034A), Zhangjiakou Science and Technology Project (Grant No. 1811036C) and Science and Technology Project of Hebei North University (Grant Nos. 220637, 12995583).

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Authors and Affiliations

  1. Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, Hebei North University, Zhangjiakou, 075000, China

    Xiuyuan Zhang, Donghao Zhang, Tongtong Zhao & Kuo He

  2. College of Food Engineering, Henan University of Animal Husbandry & Economy, Zhengzhou, 450046, China

    Lin Chen

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  1. Xiuyuan Zhang
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  2. Donghao Zhang
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  4. Lin Chen
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  5. Kuo He
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Correspondence to Kuo He.

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Zhang, X., Zhang, D., Zhao, T. et al. Development and application of a novel immunoaffinity test column assay for visual detection of cloxacillin residues in animal-derived food samples. Food Measure 14, 1884–1891 (2020). https://doi.org/10.1007/s11694-020-00435-y

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  • DOI: https://doi.org/10.1007/s11694-020-00435-y

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