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Automated pre-column derivatization with 9-xanthydrol for the determination of ethyl carbamate in food matrices by high performance liquid chromatography with fluorimetric detection

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Abstract

In this work, derivatization of ethyl carbamate (EC) with 9-xanthydrol prior to liquid chromatography with fluorimetric detection (HPLC-FLD) was revisited, focusing on refining the reaction conditions and on automation. Perchloric acid was used for sample acidification and a sequence of operations was defined to perform the reaction in the autosampler needle. Chromatographic separation together with automated pre-column derivatization accounted for 18.5 min analytical run. FLD photomultiplier gain was changed in the elution region of analyte to enhance sensitivity. The instrumental detection limit (DL) was 0.52 μg L−1; after suitable sample pretreatment, the proposed procedure was applied for EC determination in liquors, red wines and soy sauces (method DLs: 0.59; 0.96; 0.65 μg L−1, respectively). Recoveries obtained after standard addition were in the range 101–103%, 71–79% and 89–93%, respectively. Standard addition method was used for EC quantification in several samples; the concentrations ranged from not detected up to 40.7 μg L−1 in liquors, 25.9 μg L−1 in wines and 7.18 μg L−1 in soy sauces.

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Acknowledgements

The financial support from National Council of Science and Technology, Mexico (CONACYT, Project 253879), and from University of Guanajuato (Grant 051/2018), is gratefully acknowledged.

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Authors and Affiliations

  1. Chemistry Department, University of Guanajuato, L. de Retana 5, 36000, Guanajuato, Mexico

    Daniel Antonio Aguilera Ojeda, Kazimierz Wrobel, Alma Rosa Corrales Escobosa, Luis Fernando Mejia Diaz & Katarzyna Wrobel

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  1. Daniel Antonio Aguilera Ojeda
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Correspondence to Katarzyna Wrobel.

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Aguilera Ojeda, D.A., Wrobel, K., Corrales Escobosa, A.R. et al. Automated pre-column derivatization with 9-xanthydrol for the determination of ethyl carbamate in food matrices by high performance liquid chromatography with fluorimetric detection. Food Measure 13, 2722–2728 (2019). https://doi.org/10.1007/s11694-019-00193-6

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  • DOI: https://doi.org/10.1007/s11694-019-00193-6

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