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Characterization of tannins from two wild blackberries (Rubus spp) by LC–ESI–MS/MS, NMR and antioxidant capacity

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Abstract

Tannins are phenolics found in fruits such as wild Rubus berries. The aim of this study was to determine the profile by LC–ESI–MS/MS and NMR, antioxidant capacity (AOX) of the tannins in two Mexican wild blackberries. Fractions rich in tannins (FRT) were isolated using different solvents and chromatographic resins. The LC–ESI–MS/MS and NMR results showed that catechin and epicatechin (m/z 289−1), which are precursors of proanthocyanidins, as well as other six oligomeric forms of ellagitannins (m/z 301−1) were found in R. Liebmannii, whereas in R. Palmeri, only ellagitannins were found, highlighting oligomeric forms of lambertianins and sanguiins (m/z 783−1 to 1869−1). The purification pathway made it possible to increase the AOX 19.7 times from fruits to FRT showing strong positive correlations (R2 > 0.9) with total phenolic content. This study provided a good assessment of the tannin composition of wild blackberries from the Northwest of Mexico and their bioactivity as a potential edible source.

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Fig. 1

Structures adapted from Mertz et al. (2007), Kool et al. (2010), Lee et al. (2012), Melone et al. (2015), and Li et al. (2016)

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Acknowledgements

This research was supported by Universidad Autónoma de Sinaloa (Grants PROFAPI2014 and PROFAPI2015). Oscar Abel Sánchez-Velázquez’s scholarship was provided by the Consejo Nacional de Ciencia y Tecnología (CONACYT). The authors would also like to thank Dr. Alfredo Leal Orduño for his invaluable support of this research.

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Authors and Affiliations

  1. Programa Regional de Posgrado en Biotecnología, Universidad Autónoma de Sinaloa, Av. Universitarios s/n, Col. Universitarios, AP 1354, CP 80000, Culiacán Rosales, Sinaloa, Mexico

    Oscar Abel Sánchez-Velázquez, Julio Montes-Ávila, Jorge Milán-Carrillo, Cuauhtémoc Reyes-Moreno, Saraid Mora-Rochin & Edith-Oliva Cuevas-Rodríguez

  2. Posgrado en Ciencia Y Tecnología de Alimentos, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Av. Universitarios s/n, Col. Universitarios, AP 1354, CP 80000, Culiacán Rosales, Sinaloa, Mexico

    Jorge Milán-Carrillo, Cuauhtémoc Reyes-Moreno & Edith-Oliva Cuevas-Rodríguez

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  1. Oscar Abel Sánchez-Velázquez
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Correspondence to Edith-Oliva Cuevas-Rodríguez.

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Abel Sánchez-Velázquez, O., Montes-Ávila, J., Milán-Carrillo, J. et al. Characterization of tannins from two wild blackberries (Rubus spp) by LC–ESI–MS/MS, NMR and antioxidant capacity. Food Measure 13, 2265–2274 (2019). https://doi.org/10.1007/s11694-019-00146-z

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