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Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

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Abstract

Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptusbenthamii clone under different culture conditions and used canopy branches (CB) and trunk base material as explant sources. The culture media were wood plant medium (WPM), Murashige and Skoog medium (MS) and JADS (Correia and co-authors medium). Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and pH. Morphophysiology, dry mass production, endophytic manifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous development. The responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L−1 and optimal development at 30 g L−1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and pH may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation.

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Authors and Affiliations

  1. Biological Science Department, “Luiz de Queiroz” Superior College of Agriculture, University of São Paulo (ESALQ/USP), Pádua Dias Avenue, 11, P.O. Box 9, Piracicaba, SP, 13418-900, Brazil

    Natalia Pimentel Esposito-Polesi & Marcílio de Almeida

  2. Institute of Agriculture Sciences, Federal University of Minas Gerais (UFMG), Universitária Avenue, 1000, Montes Claros, MG, 39404-547, Brazil

    Leandro Silva de Oliveira

  3. State University of Goiás (UEG), Rua S7, Qd. 1, S Sul, Palmeiras de Goiás, GO, 76190-000, Brazil

    Francisco José Benedini Baccarin

  4. In vitro Palm Consultoria, Estudo e Desenvolvimento Biológico Ltda, Rua Itajobi, 421, Piracicaba, SP, 13432-000, Brazil

    Cristina Vieira de Almeida

Authors
  1. Natalia Pimentel Esposito-Polesi
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  2. Leandro Silva de Oliveira
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  3. Francisco José Benedini Baccarin
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  4. Cristina Vieira de Almeida
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  5. Marcílio de Almeida
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Correspondence to Natalia Pimentel Esposito-Polesi.

Additional information

Project funding: The work was supported by the National Council of Technological and Scientific Development (CNPq) Process No. 143253/2011-5 and to Coordination for the Improvement of Higher Education Personnel (CAPES).

The online version is available at http://www.springerlink.com

Corresponding editor: Tao Xu.

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Esposito-Polesi, N.P., de Oliveira, L.S., Baccarin, F.J.B. et al. Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources. J. For. Res. 31, 857–869 (2020). https://doi.org/10.1007/s11676-018-0816-1

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  • DOI: https://doi.org/10.1007/s11676-018-0816-1

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