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Genome engineering technologies for targeted genetic modification in plants

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Abstract

Well-established targeted technologies to engineer genomes such as zinc-finger nuclease-based editing (ZFN), transcription activator-like effector nuclease-based editing (TALEN), and clustered regularly interspaced short palindromic repeats and associated protein system-based editing (CRISPR/Cas) are proving to advance basic and applied research in numerous plant species. Compared with systems using ZFNs and TALENs, the most recently developed CRISPR/Cas system is more efficient due to its use of an RNA-guided nuclease to generate double-strand DNA breaks. To accelerate the applications of these technologies, we provide here a detailed overview of these systems, highlight the strengths and weaknesses of each, summarize research advances made with these technologies in model and crop plants, and discuss their applications in plant functional genomics. Such targeted approaches for genetically modifying plants will benefit agricultural production in the future.

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Acknowledgements

We thank our colleagues in molecular genetics and genomics who helped us during the preparation of this manuscript. We express our great thanks to Drs. M. Lauressergues, A. Omidbakhshfard, M. Page, W. Thompson, and D. Whitley for their critical reading and valuable comments that improved the manuscript.

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  1. College of Arts and Science, East Carolina University, Greenville, NC, 27858, USA

    Wei Tang

  2. University of North Carolina at Chapel Hill, 101 Stadium Drive, Chapel Hill, NC, 27514, USA

    Anna Y. Tang

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  1. Wei Tang
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Correspondence to Wei Tang.

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Corresponding editor: Chai Ruihai.

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Tang, W., Tang, A.Y. Genome engineering technologies for targeted genetic modification in plants. J. For. Res. 29, 875–887 (2018). https://doi.org/10.1007/s11676-017-0588-z

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