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Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes

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Abstract

Objective

To study the effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes, to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of Yiqi Huoxue Recipe on gene level, and to further investigate whether Yiqi Huoxue Recipe is an effective prescription for CVB3 myocarditis.

Methods

A modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between the CVB3 infection group and the treatment group with Yiqi Huoxue Recipe. The results were further verified by fluorescence RT-PCR.

Results

The results of SSH showed that the gene expression of ribosomal protein S20 in the treatment group was higher than that in the CVB3 infection group (P<0.05), which agreed with the results of fluorescent RT-PCR.

Conclusion

Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis, and Yiqi Huoxue Recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, 110032, China

    Ming-xue Zhang  (张明雪), Yu-mei Zhang  (张玉梅), Wei He  (何 伟), Hong-hua Che  (车红花) & Ping Gu  (顾 平)

  2. School of Oriental Medicine, Health Center, Life University, Marietta, USA

    Jun Esther

Authors
  1. Ming-xue Zhang  (张明雪)
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  2. Yu-mei Zhang  (张玉梅)
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  3. Jun Esther
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  4. Wei He  (何 伟)
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  5. Hong-hua Che  (车红花)
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  6. Ping Gu  (顾 平)
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Corresponding author

Correspondence to Ming-xue Zhang  (张明雪).

Additional information

Supported by National Natural Science Foundation of China (No. 30873399); Doctoral Funds from Ministry of Education (No. 20070162002); and Excellent Talents in Universities Support Plan of Liaoning Province

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Zhang, Mx., Zhang, Ym., Esther, J. et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes. Chin. J. Integr. Med. 17, 376–380 (2011). https://doi.org/10.1007/s11655-011-0729-8

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  • DOI: https://doi.org/10.1007/s11655-011-0729-8

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