Summary
Stoichiometric investigations were made for all 21 proteins of the 30S subunit and for 31 proteins of the 50S subunit. While the results for the 30S proteins strongly suggest the existence of two different stoichiometric classes, the so-called “fractional” (F) and “unit” (U) proteins with average molar amounts of 0.1–0.5 and 0.8–1.2 per mole 30S respectively, at least one further group is present in isolated 50S particles. Therefore 50S proteins may be subdivided in the following way: a) 9–10 proteins with average molar amounts of 0.2–0.6 (F-proteins), b) 15–16 proteins with 0.8–1.2 copies per 50S particle (U-proteins), c) at least one protein with an average value of 1.8–2.2 copies, the “repeated” (R) proteins, d) a group of 7 proteins with 1.4–1.7 copies per particle; they can be named “fractional repeated” (FR) proteins. These findings indicate that 50S ribosomal particles show a more differentiated degree of structural heterogeneity at least in vitro than has been shown for 30S subunits.
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References
Apirion, D.: Altered ribosomes in a suppressor strain of Escherichia coli. J. molec. Biol. 16, 285 (1966).
Craven, G. R., Gavin, R., Fanning, T.: The transfer RNA binding site of the 30S ribosome and the site of tetracycline inhibition. Cold Spr. Harb. Symp. quant. Biol. 34, 129 (1969).
Craven, G. R., Voynow, P., Hardy, S. J. S., Kurland, C. G.: The ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30S ribosomal proteins. Biochemistry 8, 2906 (1969).
Deusser, E.: Heterogeneity of ribosomal populations in Escherichia coli cells grown in different media. Molec. gen. Genet. 119, 249–258 (1972).
Duin, J. van, Knippenberg, P. H. van, Dieben, M. van, Kurland, C. G.: Functional heterogeneity of the 30S ribosomal subunit of Escherichia coli. II. Effect of S21 on initiation. Molec. gen. Genet. 116, 181 (1972).
Duin, J. van, Kurland, C. G.: Functional heterogeneity of the 30S ribosomal subunit of Escherichia coli. Molec. gen. Genet. 109, 169 (1970).
Dzionara, M., Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins. XIII: Molecular weights of isolated ribosomal proteins of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 67, 1909 (1970).
Fogel, S., Sypherd, P. S.: Chemical basis for heterogeneity of ribosomal protein. Proc. nat. Acad. Sci. (Wash.) 59, 1329 (1968).
Hardy, S. J. S., Kurland, C. G., Voynow, P., Mora, G.: The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry 8, 2897 (1969).
Hasenbank, R., Maschler, R., Stöffler, G.: Manuscript in preparation.
Hindennach, I., Kaltschmidt, E., Wittmann, H. G.: Isolation of proteins from 50S ribosomal subunits of Escherichia coli. Europ. Biochem. 23, 12 (1971b).
Hindennach, I., Stöffler, G., Wittmann, H. G.: Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunit. Europ. Biochem. 23, 7 (1971a).
Homann, H. E., Nierhaus, K. H.: Protein composition of biosynthetic precursors and artificial subparticles from ribosomal subunits in Escherichia coli K12. Europ. Biochem. 20, 249 (1971).
Kaltschmidt, E., Dzionara, M., Donner, D., Wittmann, H. G.: Ribosomal proteins. I. Isolation, amino acid composition, molecular weights and peptide mapping of proteins from Escherichia coli ribosomes. Molec. gen. Genet. 100, 364 (1967).
Kaltschmidt, E., Dzionara, M., Wittmann, H. G.: Ribosomal proteins. XV. Amino acid compositions of isolated ribosomal proteins from 30S and 50S subunits of Escherichia coli. Molec. gen. Genet. 109, 292 (1970).
Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401 (1970a).
Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc. nat. Acad. Sci. (Wash.) 67, 1276 (1970b).
Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins. XXXII. Comparison of several extraction methods for proteins from Escherichia coli ribosomes. Biochemie 54, 167 (1972).
Kischa, K., Möller, W., Stöffler, G.: Reconstitution of a GTPase activity by a 50S ribosomal protein from Escherichia coli. Nature (Lond.) 233, 62 (1971).
Kurland, C. G., Voynow, P., Hardy, S. J. S., Randall, L., Lutter, L.: Physical and functional heterogeneity of ribosomes. Cold Spr. Harb. Symb. quant. Biol. 34, 17 (1969).
Lipman, F.: Polypeptide chain elongation in protein biosynthesis. Science 164, 1024 (1969).
Möller, W., Castleman, H., Terhorst, C. P.: Characterization of an acidic protein in 50S ribosomes of Escherichia coli. FEBS Letters 8, 192 (1970).
Moore, P. B., Traut, R. R., Noller, M., Pearson, P., Delius, H.: Ribosomal proteins of Escherichia coli. II. Proteins from the 30S subunit. J. molec. Biol. 31, 441 (1968).
Mora, G., Donner, D., Thammana, P., Lutter, L., Kurland, C. G., Craven, G. R.: Purification and characterization of 50S ribosomal proteins of Escherichia coli. Molec. gen. Genet. 112, 229 (1971).
Nashimoto, H., Held, W., Kaltschmidt, E., Nomura, M.: Structure and function of bacterial ribosomes. XII. Accumulation of 21S particles by some cold-sensitive mutants of Escherichia coli. J. mol. Biol. 62, 121 (1971).
Nomura, M., Mizushima, S., Ozaki, M., Traub, P., Lowry, C. V.: structure and function of ribosomes and their molecular components. Cold Spr. Harb. Symp. quant Biol. 34, 49 (1969).
Osawa, S.: Biosynthesis of ribosomes in bacterial cells. Prog. Nucleic Acid Res. 4, 161 (1965).
Otaka, E., Itoh, T., Osawa, S.: Protein components in the 40S ribonucleoprotein particles in Escherichia coli. Science 197, 1452 (1967).
Randall-Hazelbauer, L., Kurland, C. G.: Identification of three 30S proteins contributing to the ribosomal A site. Molec. gen. Genet. 115, 234 (1972).
Retsema, J. A., Conway, T. W.: Reversible dissociation of Escherichia coli ribosomes by N-ethylmaleimide. Biochim. biophys. Acta (Amst.) 179, 369 (1969).
Roberts, R. B., Abelson, P. H., Cowie, D. B., Bolton, E. D., Britten, R. G.: Studies of biosynthesis in Escherichia coli. Kirley litographic Company, Wash. D. C. publication 607 (1963).
Sells, B. H., Davies, F. C.: Biogenesis of 50S particles in exponentially growing Escherichia coli. J. molec. Biol. 47, 155 (1970).
Stöffler, G., Wittmann, H. G.: Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins. J. molec. Biol. 62, 407 (1971).
Terhorst, C. P., Wittmann-Liebold, B., Möller, W.: 50S ribosomal proteins. Peptide studies on two acidic proteins A1 and A2, isolated from 50S ribosomes of Escherichia coli. Europ. J. Biochem. 25, 13 (1972).
Thammana, P., Weber, H. J., Deusser, E., Maschler, R., Kurland, C. G., Stöffler, G., Wittmann, H. G.: Structural and functional evidence for a repeated protein in Escherichia coli ribosomes. Nature, in press (1972).
Traut, R. A., Delius, H., Ahmad-Zadeh, C., Bickle, T. A., Pearson, P., Tissieres, A.: Ribosomal proteins of Escherichia coli: stoichiometry and implication for ribosome structure. Cold Spr. Harb. Symp. quant. Biol. 34, 25 (1969).
Traut, R. A., Moore, P. R., Delius, H., Noller, H., Tissieres, A.: Ribosomal proteins of Escherichia coli. Demonstration of different primary structures. Proc. nat. Acad. Sci. (Wash.) 57, 1294 (1967).
Voynow, P., Kurland, C. G.: Stoichiometry of the 30S ribosomal proteins of Escherichia coli. Biochemistry 10, 517 (1971).
Waller, J. P., Harris, J. I.: Studies on the composition of the protein from Escherichia coli. ribosomes. Proc. nat. Acad. Sci. (Wash.) 47, 18 (1961).
Wittmann, H. G., Stöffler, G., Hindennach, I., Kurland, C. G., Randall-Hazelbauer, L., Birge, E. A., Nomura, M., Kaltschmidt, E., Mizushima, S.: Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories. Mol. gen. Genet. 111, 327 (1971).
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Communicated by P. Starlinger
Paper No. 35 on “Ribosomal Proteins”. Preceding paper is by E. Deusser and H. G. Wittmann, Nature 238, 269 (1972).
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Weber, H.J. Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli . Molec. gen. Genet. 119, 233–248 (1972). https://doi.org/10.1007/BF00333861
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DOI: https://doi.org/10.1007/BF00333861