Summary
Regeneration of several varieties of soybean [Glycine max (L.) Merrill] by somatic embryogenesis from cultured epicotyls and primary leaves has been demonstrated. Somatic embryogenesis was induced from epicotyls and primary leaves when cotyledon halves with the intact zygotic embryo axes were cultured on Murashige and Skoog (MS) medium supplemented with 10 mg 1−1 (45.2 µM) 2,4-D. Stable, continuously proliferating globular embryo cultures (GEC) were established from small groups of somatic embryos on MS medium supplemented with 20 mg 1−1 (90.5 µM) 2,4-dichlorophenoxyacetic acid (2,4-D). Rapid multiplication of shoot tips from germinating somatic embryos was achieved on Cheng’s basal medium (CBO) containing 2.5 mg 1−1 (11.3 µM) 6-benzyladenine. Fertile plants were obtained from individual somatic embryos and in vitro propagated adventitious shoot bud cultures.
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Rajasekaran, K., Pellow, J.W. Somatic embryogenesis from cultured epicotyls and primary leaves of soybean [Glycine max (L.) Merrill]. In Vitro Cell.Dev.Biol.-Plant 33, 88–91 (1997). https://doi.org/10.1007/s11627-997-0002-1
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DOI: https://doi.org/10.1007/s11627-997-0002-1