Skip to main content
SpringerLink
Log in

Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

A method for the induction of somatic embryos in soybean tissue cultures is described. Cotyledons from immature embryos were utilized as explant source. Supplementing the culture medium with auxins (2,4-D, MCPA, 2,4,5-T, NAA, IAA, IBA) caused formation of meristematic tissue on cotyledon explants. The extent of meristematic tissue formed depended on the kind and concentration of auxin in the culture medium. With 2,4-D and MCPA, embryoids originated from meristematic tissue. Embryoid formation rates were influenced by the developmental stage of the embryos serving as explant source and auxin concentration. Addition of cytokinins to the medium containing 2,4-D or supplementing it with high sugar concentrations inhibited the formation of meristematic tissue and of embryoids on cotyledon explants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenin

2,4-D:

2,4-dichlorphenoxyacetic acid

IAA:

indoleacetic acid

IBA:

indolebutyric acid

MCPA:

2-methyl-4-chlorophenoxyacetic acid

NAA:

∝-naphthaleneacetic acid

2,4,5-T:

2,4,5-trichlorophenoxyacetic acid

L2:

Phillips and Collins (1979) medium

References

  • Beversdorf WD, Bingham ET (1977) Crop Sci 17: 307–311

    Google Scholar 

  • Cheng TY, Saka H, Voqui-Dinh TH (1980) Plant Sci Lett 19: 91–99

    Google Scholar 

  • Gamborg OL, Davis BP, Stahlhut RW (1983) Plant Cell Reports 2: 213–215

    Google Scholar 

  • Kartha KK, Pahl K, Leung NL, Mroginski LA (1981) Can J Bot 59: 1671–1679

    Google Scholar 

  • Phillips GC, Collins GB (1979) Crop Sci 19: 59–64

    Google Scholar 

  • Phillips GC, Collins GB (1981) Plant Cell Tissue Organ Culture 1: 123–129

    Google Scholar 

  • Saka H, Voqui-Dinh TH, Cheng TY (1980) Plant Sci Lett 19: 193–201

    Google Scholar 

  • Widholm JM, Rick S (1983) Plant Cell Reports 2: 19–20

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Züchtungsforschung Quedlinburg, Akademie der Landwirtschaftswissenschaften der DDR, DDR-4300, Quedlinburg

    Bärbel Lippmann & Gunter Lippmann

Authors
  1. Bärbel Lippmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gunter Lippmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by D. von Wettstein

Present and correspondence address: Akademie der Landwirtschaftswissenschaften der DDR, Institut für Pflanzenernährung, DDR-6909, Jena

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lippmann, B., Lippmann, G. Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr. Plant Cell Reports 3, 215–218 (1984). https://doi.org/10.1007/BF00269295

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269295

Keywords

Search

Navigation