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Direct organogenesis from cortical cells of hypocotyl segments in soybean

  • Plant Tissue Culture
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Abstract

This study describes direct organogenesis from soybean hypocotyl segments with distinctions of adventitious shoot differentiation without callus phase along with high frequency of plantlet development (64.04%) within a period of 14 wk. This was mainly attributed to manipulation of the culture medium with potent plant growth regulators. A large number of segments (77.36 ± 1.01%) cultured on medium fortified with 1.0 mg L−1 TDZ displayed appearance of adventitious buds on acropetal ends in 15 d with 2.67 ± 0.58 buds per segment. The shoot bud-bearing segments (64.10 ± 4.44%) exhibited 3.67 ± 0.58 shoots per segment on medium containing 2.0 mg L−1 AgNO3 within 15 d of culturing. The transfer of multiple shoots on elongation medium resulted in shoot elongation within 15 d. A sizeable number of elongated shoots (87.02 ± 3.01%) showed adventitious root induction with 3.33 ± 1.15 roots per shoot on medium supplemented with 3.0 mg L−1 IBA in 6 wk. The plantlets were hardened successfully with a survival rate of 94.89% in soil and demonstrated normal growth. The histological analysis revealed that leafy shoot-like structures originated from the cortical region of hypocotyl segments confirming a phenomenon of direct shoot regeneration.

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All data generated or analysed during this study are included in this article.

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Acknowledgements

The authors thank the School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, for providing infrastructural support to carry out this research.

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Authors and Affiliations

  1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India

    Ekta Patel, Ajinder Kaur & Jagdeep Singh Sandhu

  2. Electron Microscopy and Nanoscience Laboratory, Punjab Agricultural University, Ludhiana, 141 004, India

    Anu Kalia

  3. Department of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, 141 004, India

    Balwinder Singh Gill

Authors
  1. Ekta Patel
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  2. Anu Kalia
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  3. Balwinder Singh Gill
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  4. Ajinder Kaur
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  5. Jagdeep Singh Sandhu
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Contributions

EP conducted in vitro experiments. AKalia carried out histological analysis. BSG provided the seed material and maintained plants in the glasshouse. AKaur supervised in vitro experiments and analyzed the direct organogenesis data. JSS planned, coordinated the experiments, interpreted results, and drafted the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Jagdeep Singh Sandhu.

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The authors declare no competing interests.

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Patel, E., Kalia, A., Gill, B.S. et al. Direct organogenesis from cortical cells of hypocotyl segments in soybean. In Vitro Cell.Dev.Biol.-Plant 59, 140–146 (2023). https://doi.org/10.1007/s11627-023-10329-5

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  • DOI: https://doi.org/10.1007/s11627-023-10329-5

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