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Cryopreservation of somatic embryos from Petiveria alliacea L. by different techniques based on vitrification

  • Plant Tissue Culture
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Abstract

Petiveria alliacea L. is a medicinal plant originating from the Amazon region. This study describes an efficient cryopreservation protocol for somatic embryos (SEs) produced from roots of P. alliacea based on the comparison of vitrification, encapsulation-dehydration, and D cryo-plate techniques. With the vitrification technique, SEs treated with PVS2 solution (0.4 M sucrose, 3.3 M glycerol, 2.4 M ethylene glycol, and 1.9 M DMSO) for 30 min displayed high viability (85%) and intermediate proliferation recovery (about 12 adventitious SEs produced from original SEs [SEs/SE] after 90 d of culture). With the encapsulation-dehydration technique, lower viability (70%) and very low proliferation recovery (about two SEs/SE) were achieved with cryopreserved SEs dehydrated for 10 min in a laminar air flow cabinet. The D cryo-plate technique led to high viability (85%) and proliferation recovery (19 SEs/SE) of cryopreserved SEs after 90 min dehydration. In the experimental conditions tested, the D cryo-plate method was the most efficient technique for cryopreservation of P. alliacea SEs.

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Acknowledgments

The authors are grateful to the Coordination of Improvement of Higher Education Personnel (CAPES/Brazil) and to the National Council for Scientific and Technological Development (CNPq/Brazil) for funding an international collaborative research project (Programme Science without Borders) between UERJ (University of the State of Rio de Janeiro) and IRD (Institut de Recherche pour le Développement). This study was also supported by the Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ).

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Authors and Affiliations

  1. Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier 524 – PHLC sala 602 – Maracanã, Rio de Janeiro, CEP 20550-900, Brazil

    Jamine de Almeida Pettinelli, Bianka de Oliveira Soares, Leila Cantelmo, Renata de Oliveira Garcia, Elisabeth Mansur & Rachel Fatima Gagliardi

  2. Institut de Recherche pour le Développement (IRD), 911 avenue Agropolis, BP 64501, 34394, Montpellier Cedex 5, France

    Florent Engelmann

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  1. Jamine de Almeida Pettinelli
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  2. Bianka de Oliveira Soares
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  3. Leila Cantelmo
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  4. Renata de Oliveira Garcia
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  5. Elisabeth Mansur
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  6. Florent Engelmann
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  7. Rachel Fatima Gagliardi
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Correspondence to Rachel Fatima Gagliardi.

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Editor: Barbara Reed

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de Almeida Pettinelli, J., de Oliveira Soares, B., Cantelmo, L. et al. Cryopreservation of somatic embryos from Petiveria alliacea L. by different techniques based on vitrification. In Vitro Cell.Dev.Biol.-Plant 53, 339–345 (2017). https://doi.org/10.1007/s11627-017-9820-y

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  • DOI: https://doi.org/10.1007/s11627-017-9820-y

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