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Light quality affects in vitro growth and essential oil profile in Lippia alba (Verbenaceae)

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Abstract

This study evaluated the influence of light quality on growth and essential oil production in three chemotypes of Lippia alba (BGEN-01, BGEN-02, and BGEN-42) grown in vitro. These chemotypes differed both in ploidy and in the predominant essential oil produced. The treatments were fluorescent lamps, white light-emitting diode (LED) bulbs, and blue/red LEDs, all with the same irradiance. After 40 d of culture on hormone-free MS medium, analysis of the essential oil profile and quantitative analysis of growth parameters—plant height, fresh, and dry weight, total chlorophyll, and total carotenoids—were performed. Light quality significantly influenced the in vitro growth of L. alba. Compared to the control, blue/red LEDs induced greater fresh and dry weight in BGEN-01 and BGEN-02 and less in BGEN-42. Photosynthetic pigment levels were higher in plants grown under blue/red LEDs for all chemotypes. Multivariate analysis allowed the identification of different patterns of essential oil production among the treatments. The composition of the volatile compounds varied with light quality and chemotype, with the differences due mainly to the amounts of eucalyptol and linalool. The knowledge of this relationship between light quality and essential oil profile provides a basis for further studies at the genetic level that may elucidate how this regulation works, thereby enabling tailored production of compounds of interest.

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References

  • Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry, 4th edn. Allured Publishing, Carol Stream

    Google Scholar 

  • Agência Nacional de Vigilância Sanitária (2010) Farmacopeia Brasileira, 5th edn. Anvisa, Brasília, pp 198–199

    Google Scholar 

  • Aguiar JS, Costa MCCD, Nascimento SC, Sena KXFR (2008) Atividade antimicrobiana de Lippia alba (Mill.) N. E. Brown (Verbenaceae). Rev Bras Farm 18:436–440

    Article  Google Scholar 

  • Ahmad N, Rab A, Ahmad N (2016) Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert). J Photochem Photobiol B 154:51–56

    Article  CAS  PubMed  Google Scholar 

  • Alvarenga ICA, Pacheco FV, Silva ST, Bertolucci SKV, Pinto JEBP (2015) In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles. Plant Cell Tissue Organ Cult 122:299–308

    Article  CAS  Google Scholar 

  • Braun DM, Ma Y, Inada N, Muszynski MG, Baker RF (2006) tie-dyed1 regulates carbohydrate accumulation in maize leaves. Plant Physiol 142:1511–1522

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Carmona F, Angelucci MA, Salesb DS, Chiarattib TM, Honoratoa FB, Bianchi RV, Pereira MAS (2013) Lippia alba (Mill.) N. E. Brown hydroethanolic extract of the leaves is effective in the treatment of migraine in women. Phytomedicine 20:947–950

    Article  PubMed  Google Scholar 

  • Core Team R (2014) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna

    Google Scholar 

  • Friendly M, Fox J (2013) Visualizing generalized canonical discriminant and canonical correlation analysis. Documentation for R Package “candisc”, version 0.6–5 http://CRAN.R-project.org/package=candisc.

  • Golovatskaya IF, Karnachuk RA (2015) Role of green light in physiological activity of plants. Russ J Plant Physiol 62:727–740

    Article  CAS  Google Scholar 

  • Gupta SD, Jatothu B (2013) Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechnol Rep 7:211–220

    Article  Google Scholar 

  • Hennebelle T, Sahpaz S, Joseph H, Bailleul F (2008) Ethnopharmacology of Lippia alba. J Ethnopharmacol 116:211–222

    Article  CAS  PubMed  Google Scholar 

  • Heo JW, Lee CW, Paek KY (2006) Influence of mixed LED radiation on the growth of annual plants. J Plant Biol 49:286–290

    Article  Google Scholar 

  • Hoffman EW, Miller M, Louw E-L (2015) The efficacy of LED lights and growth regulator sprays at controlling chilling injury in Leucospermum potted plants. Acta Hortic 1097:47–54

    Article  Google Scholar 

  • Hogewoning SW, Wientjes E, Douwstra P, Trouwborst G, VanIeperen W, Croce R, Harbinson J (2012) Photosynthetic quantum yield dynamics: from photosystems to leaves. Plant Cell 24:1921–1935

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kalaji HM, Schansker G, Ladle RJ, Goltsev V, Bosa K, Allakhverdiev SI, Brestic M, Bussotti F, Calatayud A, Dabrowski P, Elsheery NI, Ferroni L, Guidi L, Hogewoning SW, Jajoo A, Misra AN, Nebauer SG, Pancaldi S, Penella C, Poli DB, Pollastrini M, Romanowska-Duda ZB, Rutkowska B, Seródio J, Suresh K, Szulc W, Tambussi E, Yanniccari M, Zivcak M (2014) Frequently asked questions about in vivo chlorophyll fluorescence: practical issues. Photosynth Res 122:121–158

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143–151

    Article  Google Scholar 

  • Kurepin LV, Pharis RP, Emery RJN, Reid DM, Chinnappa CC (2015) Phenotypic plasticity of sun and shade ecotypes of Stellaria longipes in response to light quality signaling, gibberellins and auxin. Plant Physiol Biochem 94:174–180

    Article  CAS  PubMed  Google Scholar 

  • Kurilčik A, Miklušytė-Čanova R, Dapkūnienė S, Žilinskaitė S, Kurilčik G, Tamulaitis G, Duchovskis P, Žukauskas A (2008) In vitro culture of Chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 3:161–167

    Google Scholar 

  • Lin Y, Li J, Li B, He T, Chun Z (2011) Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell Tissue Organ Cult 105:329–335

    Article  Google Scholar 

  • Massa GD, Kim HH, Wheeler RM, Mitchell CA (2008) Plant productivity in response to LED lighting. Hortic Sci 43:1951–1956

    Google Scholar 

  • Mjos SA, Meier S, Boitsov S (2006) Alkylphenol retention indices. J Chromatogr 1123:98–105

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tissue Organ Cult 73:43–52

    Article  CAS  Google Scholar 

  • Oliveira GT, Ferreira JM, Rosa LH, Siqueira EP, Johann S, Lima LA (2014) In vitro antifungal activities of leaf extracts of Lippia alba (Verbenaceae) against clinically important yeast species. Rev Soc Bras Med Trop 47:247–250

    Article  PubMed  Google Scholar 

  • Pandeló D, Melo TD, Singulani JL, Guedes FAF, Machado MA, Coelho CM, Viccini LF, Santos MO (2012) Oil production at different stages of leaf development in Lippia alba. Braz J Pharmacog 22:497–501

    Google Scholar 

  • Park YG, Oh HJ, Jeong BR (2013) Growth and anthocyanin concentration of Perilla frutescens var. acuta Kudo as affected by light source and DIF under controlled environment. Hortic Environ Biotechnol 54:103–108

    Article  CAS  Google Scholar 

  • Poudel PR, Kataoka I, Mochioka R (2008) Effect of red-and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tissue Organ Cult 92:147–153

    Article  Google Scholar 

  • Saebo A, Krekling T, Appelgren M (1995) Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tissue Organ Cult 41:177–185

    Article  Google Scholar 

  • Sáez PL, Bravo LA, Latsague MI, Toneatti MJ, Sánchez-Olate M, Ríos DG (2013) Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition. Plant Sci 201:12–24

    Article  PubMed  Google Scholar 

  • Schuerger AC, Brown C, Stryjewski EC (1997) Anatomical features of pepper plants (Capsicum annuum L.) growth under red light emitting diodes supplemented with blue or far-red light. Ann Bot 79:273–282

    Article  CAS  PubMed  Google Scholar 

  • Scott RJ, Knott M (1974) A cluster analysis method for grouping mans in the analysis of variance. Biometrics 30:507–512

    Article  Google Scholar 

  • Sims DA, Pearcy RW (1992) Response of leaf anatomy and photosynthetic capacity in Alocasia macrorrhiza (Araceae) to a transfer from low to high light. Am J Bot 79:449–455

    Article  Google Scholar 

  • Soussi M, Ocana A, Lluch C (1998) Effects of salt stress on growth, photosynthesis and nitrogen fixation in chickpea (Cicer arietinum L.). J Exp Bot 49:1329–1337

    Article  CAS  Google Scholar 

  • Viccini LF, Silveira RS, do Vale AA (2014) Citral and linalool content has been correlated to DNA content in Lippia alba (Mill.) N.E. Brown (Verbenaceae). Ind Crop Prod 59:14–19

    Article  CAS  Google Scholar 

  • Wang XY, Xu XM, Cui J (2015) The importance of blue light for leaf area expansion, development of photosynthetic apparatus, and chloroplast ultrastructure of Cucumis sativus grown under weak light. Photosynthetica 53:213–222

    Article  CAS  Google Scholar 

  • Yeater KM, Duke SE, Riedell WE (2015) Multivariate analysis: greater insights into complex systems. Agron J 107:799–810

    Article  Google Scholar 

  • Yeh H, Chung JP (2009) High-brightness LEDs—energy efficient lighting sources and their potential in indoor plant cultivation. Renew Sust Energ Rev 13:2175–2180

    Article  CAS  Google Scholar 

  • Yu KW, Murthy HN, Hahn EJ, Paek KY (2005) Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality. Biochem Eng J 23:53–56

    Article  CAS  Google Scholar 

  • Zienkiewicz M, Drozak A, Wasilewska W, Bacławska I, Przedpełska-Wasowicz E, Romanowska (2015) The short-term response of Arabidopsis thaliana (C3) and Zea mays (C4) chloroplasts to red and far red light. Planta 242:1479–1493

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank the Brazilian sponsoring agencies, CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil), FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior), for financial support. We also acknowledge the Gas Chromatography Mass Spectrometry Laboratory—CGMSLab, CAPQ/UFLA for the qualitative analyses of essential oils.

Authors’ contributions

DSB, KMC, and MGC performed the experiments; DSB and KMC raised the in vitro plants for the experiments; MLT, TAS, LIS, DSB, and MGC performed extraction and analyses of essential oils; DSB, ARS, MOS, LFV, MGC, and WCO contributed to the design and interpretation of the research and to the writing of the paper.

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Authors and Affiliations

  1. Laboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Av. P.H. Rolfs s/n, Campus Universitário, 36570-000, Viçosa, MG, Brazil

    Diego Silva Batista, Kamila Motta de Castro & Wagner Campos Otoni

  2. Instituto Federal Goiano, Campus Urutaí, Urutaí, GO, Brazil

    Anderson Rodrigo da Silva

  3. Laboratório de Óleos Essenciais, Departamento de Química, Universidade Federal de Lavras, Lavras, MG, Brazil

    Maria Luisa Teixeira, Thaís Aparecida Sales, Luana Isac Soares & Maria das Graças Cardoso

  4. Laboratório de Genética, Departamento de Biologia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil

    Marcelo de Oliveira Santos & Lyderson Facio Viccini

  5. Plant Biology Department, Plant Tissue Culture Laboratory/BIOAGRO, Federal University of Viçosa, University Campus, P.H. Rolfs Avenue, 36570-000, Viçosa, MG, Brazil

    Wagner Campos Otoni

Authors
  1. Diego Silva Batista
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  2. Kamila Motta de Castro
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  3. Anderson Rodrigo da Silva
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  4. Maria Luisa Teixeira
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  5. Thaís Aparecida Sales
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  7. Maria das Graças Cardoso
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  8. Marcelo de Oliveira Santos
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  9. Lyderson Facio Viccini
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  10. Wagner Campos Otoni
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Corresponding author

Correspondence to Wagner Campos Otoni.

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Editor: David Duncan

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Batista, D.S., de Castro, K.M., da Silva, A.R. et al. Light quality affects in vitro growth and essential oil profile in Lippia alba (Verbenaceae). In Vitro Cell.Dev.Biol.-Plant 52, 276–282 (2016). https://doi.org/10.1007/s11627-016-9761-x

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  • DOI: https://doi.org/10.1007/s11627-016-9761-x

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