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Cryopreservation of in vitro-grown shoot tips of Cleome rosea Vahl (Cleomaceae) using the V cryo-plate technique

  • Plant Tissue Culture
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Abstract

This report highlights the first successful cryopreservation of in vitro shoot tips of Cleome rosea, achieved by the vitrification technique using aluminum cryo-plates (V cryo-plate). The effects on survival and recovery of C. rosea shoot tips of different plant vitrification solutions (PVS2 and PVS3), light intensity (standard and dim light), and 6-benzyladenine (BA) supplementation (0.10, 0.25, or 0.50 mg L−1 for 1 or 3 wk) in the recovery medium were investigated. Cryopreserved shoot tips showed high regeneration frequencies when treated with PVS2 and PVS3, reaching survival frequencies of 97 and 70%, respectively. When placed onto medium without growth regulators, recovery of cryopreserved shoot tips did not exceed 33% (obtained with PVS2) or 23% (PVS3). Supplementation of recovery medium with 0.5 mg L−1 BA for 3 wk increased both survival and recovery to 100% after cryopreservation following treatment with either PVS2 or PVS3. These values were maintained even when shoot tips were cultured in the presence of 0.10 mg L−1 BA for 1 wk. Maintenance under dim light resulted in better phenotypic characteristics of plants produced from cryopreserved shoot tips.

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Acknowledgments

This work was supported by the Coordination of Improvement of Higher Education Personnel (CAPES/Brazil) and the National Council of Technological and Scientific Development (CNPq/Brazil) through an international collaborative research project (Programme Science without Borders—project no. A054/2013) between the Plant Biotechnology Center of the Rio de Janeiro State University (UERJ/Brazil) and the Institut de Recherche pour le Développement (IRD/Montpellier, France). The work was also supported by the Rio de Janeiro State Foundation to Support Research (FAPERJ). The authors thank Hugo Ricardo Secioso Santos (UERJ) for help with export permits and Isabelle Engelmann-Sylvestre (IRD) for laboratory assistance.

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Authors and Affiliations

  1. Núcleo de Biotecnologia Vegetal (NBV), Instituto de Biologia Roberto Alcantara Gomes (IBRAG), Universidade do Estado do Rio de Janeiro (UERJ), Rua São Francisco Xavier, 524, PHLC, sala 509, CEP 20550-013, Rio de Janeiro, RJ, Brazil

    Lívia da Silva Cordeiro, Claudia Simões-Gurgel & Norma Albarello

  2. UMR DIADE, Institut de Recherche pour le Développement (IRD), 911 Avenue Agropolis, BP 64501, 34394, Montpellier, France

    Florent Engelmann

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  1. Lívia da Silva Cordeiro
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  2. Claudia Simões-Gurgel
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  3. Norma Albarello
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  4. Florent Engelmann
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Correspondence to Lívia da Silva Cordeiro.

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Editor: David Duncan

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da Silva Cordeiro, L., Simões-Gurgel, C., Albarello, N. et al. Cryopreservation of in vitro-grown shoot tips of Cleome rosea Vahl (Cleomaceae) using the V cryo-plate technique. In Vitro Cell.Dev.Biol.-Plant 51, 688–695 (2015). https://doi.org/10.1007/s11627-015-9714-9

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