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Induction of adventitious shoot regeneration in chrysanthemum as affected by the season

  • Plant Tissue Culture
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Abstract

The subject of investigation was Chrysanthemum x grandiflorum (Ramat.) Kitam., ‘Satinbleu’. Leaf explants and internodes were excised from the middle part of the donor sterile plant. Two methods of explant inoculation were applied: explants were placed polarly and horizontally. Modified MS medium (Murashige and Skoog 1962), supplemented with 11.4 μM indoleacetic acid and 2.7 μM 6-benzylaminopurine, were prepared to induce adventitious bud formation. Four dates of explant inoculation on the medium were tested: January 15, April 15, July 15, and October 15. Thus, regeneration occurred in winter, spring, summer, and autumn. In the present study, a more intensive regeneration in ‘Satinbleu’ chrysanthemum was observed in summer and autumn than in spring and winter, irrespective of the kind of explant and the inoculation method.

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Authors and Affiliations

  1. Laboratory of Biotechnology, Department of Ornamental Plants and Vegetable Crops, University of Technology and Life Sciences, Bernardyńska 6, PL-85-029, Bydgoszcz, Poland

    Małgorzata Zalewska, Justyna Lema-Rumińska, Natalia Miler, Magdalena Gruszka & Weronika Dąbal

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  1. Małgorzata Zalewska
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  2. Justyna Lema-Rumińska
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  3. Natalia Miler
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  4. Magdalena Gruszka
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  5. Weronika Dąbal
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Correspondence to Justyna Lema-Rumińska.

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Editor: M. Cheng

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Zalewska, M., Lema-Rumińska, J., Miler, N. et al. Induction of adventitious shoot regeneration in chrysanthemum as affected by the season. In Vitro Cell.Dev.Biol.-Plant 47, 375–378 (2011). https://doi.org/10.1007/s11627-010-9330-7

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  • DOI: https://doi.org/10.1007/s11627-010-9330-7

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