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Development of a temporary immersion system (RITA®) for mass production of sugarcane (Saccharum spp. interspecific hybrids)

  • Micropropagation
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Abstract

Commercial micropropagation of sugarcane is largely determined by the clonal fidelity and the cost of plants produced. Rapid production of plants in vitro reduces the frequency of offtypes in many species. By exploiting the concept of transverse thin cell layer culture, we have developed a rapid, high frequency direct plant regeneration system, called SmartSett®, for commercial sugarcane cultivars grown in Australia. Similar to conventional micropropagation, labour remains the major cost of this plant production system. Hence, to reduce the labour component, we have integrated the SmartSett® system with the RITA® temporary immersion bioreactor. Thin transverse leaf sections or fragmented leaves cultured on agar-based SmartSett® shoot induction medium were used as the starting material for RITA®. Shoot initiation on semi-solid medium prior to transferring to RITA®, culture immersion frequency, explant size and genotype determined the productivity (number of plants produced per unit culture) of the system. Results obtained with cultivar Q165 indicate that explants cultured for 45 d on SmartSett® shoot induction medium were the most prolific, producing on average 275 shoots per vessel after 45 d of culture in RITA with 1 min immersion every 12 or 24 h. Using the fragmented tissue, 14-d-old explants and 3-mm leaf tissue fragments were the most productive. Experiments with three cultivars (Q117, Q165 and Q205) showed that RITA® culture conditions need to be optimised for each cultivar for maximum plant production.

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Acknowledgements

We thank Clem Kuek for his invaluable critique of the original manuscript, Namie Patterson for her support and technical assistance and Ann Rizzo, Rhylee Swain and Jeff Smith for providing plant material for this research. For statistical knowledge and help, we thank Del Greenway and Jo Stringer.

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Authors and Affiliations

  1. David North Plant Research Centre, BSES Limited, P.O. Box 86, Indooroopilly, Brisbane, QLD, 4068, Australia

    Angela M. Mordocco, Jean A. Brumbley & Prakash Lakshmanan

Authors
  1. Angela M. Mordocco
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  2. Jean A. Brumbley
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  3. Prakash Lakshmanan
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Correspondence to Angela M. Mordocco.

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Editor: Gregory C. Phillips

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Mordocco, A.M., Brumbley, J.A. & Lakshmanan, P. Development of a temporary immersion system (RITA®) for mass production of sugarcane (Saccharum spp. interspecific hybrids). In Vitro Cell.Dev.Biol.-Plant 45, 450–457 (2009). https://doi.org/10.1007/s11627-008-9173-7

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