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Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume

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Abstract

This study describes a reproducible protocol for rapid mass propagation of a multipurpose legume, Clitoria ternatea L., using cotyledonary node explants derived from axenic seedlings. Multiple shoots were induced in Murashige and Skoog (MS) medium supplemented with N 6-benzyladenine (BA), zeatin riboside, or thidiazuron. N 6-Benzyladenine at 1.0 mg l−1 (4.44 μM) was most effective for shoot proliferation. Multiple shoots were also induced in nodal segments of in vitro-raised shoots grown on MS medium containing 1.0 mg l−1 (4.44 μM) of BA. Rooting was best induced in shoots grown on half-strength MS medium with 0.25 mg l−1 (1.42 μM) of indole-3-butyric acid. Plants were acclimatized in vermicompost and established in soil where they flowered and formed mature seeds.

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References

  • Banerjee, S.; Zehra, M.; Kumar, S. In vitro multiplication of Centella asiatica, a medicinal herb from leaf explants. Curr. Sci. 76: 147–148; 1999.

    Google Scholar 

  • Barik, D. P.; Naik, S. K.; Mohapatra, U.; Chand, P. K. High frequency plant regeneration by in vitro shoot proliferation in cotyledonary node explants of grasspea (Lathyrus sativus L.). In Vitro Cell. Dev. Biol. Plant. 40: 467–470; 2004.

    Article  CAS  Google Scholar 

  • Bhuyan, A. K.; Pattnaik, S. K.; Chand, P. K. Micropropagation of curry leaf tree (Murraya koenigii (L.) Spreng.) by axillary proliferation using intact seedlings. Plant Cell Rep. 16: 779–782; 1997.

    Article  CAS  Google Scholar 

  • Collen, A. M. C.; Jarl, C. I. Comparison of different methods for plant regeneration and transformation of the legume Galega orientalis Lam. Plant Cell Rep. 19: 13–19; 1999.

    Article  CAS  Google Scholar 

  • Dhanalakshmi, S.; Lakshmanan, K. K. In vitro somatic embryogenesis and plant regeneration in Clitoria ternatea. J. Exp. Bot. 43: 213–219; 1992.

    Article  Google Scholar 

  • Faisal, M.; Ahmad, N.; Anis, M. Shoot multiplication in Rauvolfia tetraphulla L. using thidiazuron. Plant Cell Tiss. Organ Cult. 80: 87–90; 2005.

    Article  CAS  Google Scholar 

  • Faisal, M.; Anis, M. Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. Plant Cell Tiss. Organ Cult. 75: 125–129; 2003.

    Article  CAS  Google Scholar 

  • Gomez, K. A.; Gomez, A. A. Statistical procedure for agricultural research. New York: John Wiley & Sons; 1984.

    Google Scholar 

  • Gulati, A.; Jaiwal, P. K. Plant regeneration from cotyledonary node explants of mungbean [Vigna radiata (L.) Wilczek]. Plant Cell Rep. 13: 523–527; 1994.

    Article  CAS  Google Scholar 

  • Jain, N. N.; Ohal, C. C.; Shroff, S. K.; Bhutada , R. H., Somani, R. S.; Kasture, S. B. Clitoria ternatea and CNS. Pharmacol. Biochem. Behav. 75: 529–536; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Kalamani, A.; Michael Gomez, S. In vitro propagation studies in Clitoria spp. Int. J. Mendel. 19: 29–30; 2002.

    Google Scholar 

  • Kaneda, Y.; Tabei, Y.; Nishimura, S.; Harada, K.; Akihama, T.; Kitamura, K. Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybean [Glycine max (L.) Merr.]. Plant Cell Rep. 1: 8–12; 1997.

    Article  Google Scholar 

  • Kelemu, S.; Cardona, C.; Segura, G. Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea, a tropical forage legume. Plant Physiol. Biochem. 42: 867–873; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Khalafalla, M. M.; Hattori, K. A combination of thidiazuron and benzyladenine promotes multiple shoot production from cotyledonary node explants of faba bean (Vicia faba L.). Plant Growth Regul. 27: 145–148; 1999.

    Article  CAS  Google Scholar 

  • Lakshmanan, K. K.; Dhanalakshmi, S. Callus, organogenesis and plantlet formation in tissue cultures of Clitoria ternatea. Ann. Bot. 66: 451–455; 1990.

    Google Scholar 

  • Malabadi, R. B.; Nataraja, K. Shoot regeneration in leaf explants of Clitoria ternatea L. cultured in vitro. Phytomorphology 51: 169–171; 2001.

    Google Scholar 

  • Michael Gomez, S. M.; Kalamani, A. Butterfly pea (Clitoria ternatea L.): A nutritive multipurpose forage legume for the tropics—An overview. Pakistan J. Nutr. 2(6): 374–379; 2003.

    Article  Google Scholar 

  • Morris, J. B. Legume genetic resources with novel “value added” industrial and pharmaceutical use. In: Janick, J. (ed.). Prospective on New crops and New Uses. Alexandria, VA. Ashs Press; 1999: 196–201.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 472–497; 1962.

    Article  Google Scholar 

  • Naik, S. K.; Pattnaik, S.; Chand, P. K. High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.). Sci. Hortic. 85: 261–270; 2000.

    Article  CAS  Google Scholar 

  • Parimala Devi, B.; Boominathan, R.; Mandal, S. C. Anti-inflammatory, analgesic and antipyretic properties of Clitoria ternatea root. Fitoterapia 74: 345–349; 2003.

    PubMed  Google Scholar 

  • Polisetty, R.; Paul, V.; Deveshwar, J. J.; Khetarpal, S.; Suresh, K.; Chandra, R. Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chick pea (Cicer arietinum L.). Plant Cell Rep. 16: 565–571; 1997.

    CAS  Google Scholar 

  • Pradhan, C.; Kar, S.; Pattnaik, S. K.; Chand, P. K. Propagation of Dalbergiasissoo Roxb. through in vitro shoot proliferation from cotyledonary nodes. Plant Cell. Rep. 18: 122–126; 1998a.

    Article  CAS  Google Scholar 

  • Pradhan, C.; Pattnaik, S. K.; Chand, P. K. Rapid in vitro propagation of East Indian rosewood (Dalbergia latifolia Roxb) through high frequency shoot proliferation from cotyledonary nodes. J. Plant Biochem. Biotechnol. 7: 61–64; 1998b.

    CAS  Google Scholar 

  • Rout, G. R. Effect of cytokinins and auxins on micropropagation of Clitoria ternatea L. Biol. Lett. 41: 21–26; 2004.

    CAS  Google Scholar 

  • Rout, G. R. Micropropagation of Clitoria ternatea Linn. (Fabaceae)—An important medicinal plant. In Vitro Cell. Dev. Biol. Plant 41: 516–519; 2005.

    Article  Google Scholar 

  • Sahoo, Y.; Pattnaik, S. K.; Chand, P. K. In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (Sweet basil) by axillary shoot proliferation. In Vitro Cell. Dev. Biol. Plant 33: 293–296; 1997.

    Google Scholar 

  • Singh, N. D.; Sahoo, L.; Sonia.; Jaiwal, P. K. In vitro shoot organogenesis and plant regeneration from cotyledonary node and leaf explants of pigeon pea (Cajanus cajan L. Mill sp). Physiol. Mol. Biol. Plant 8: 133–140; 2002.

    Google Scholar 

  • Taranalli, A. D.; Cheeramkuzhy, T. C. Influence of Clitoria ternatea extracts on memory and cerebro cholinergic activity in rats. Pharm. Biol. 38: 51–56; 2003.

    Article  Google Scholar 

  • The Wealth of India: A Dictionary of Indian Raw Materials and Industrial Products. Publication and Information Directorate, CSIR, New Delhi, India. Vol II: 1988.

  • Warrier, P. K.; Nambiar, V. P. K.; Ramankutty, C. (Eds) Indian Medicinal Plants, vol 2. Hyderabad, India: Orient Longman Limited; 1994: 129–132.

  • Yoganarasimhan, S. N. Medicinal plants of India. vol. 2, Bangalore, India: Interline Publishing Co.; 2000: 146–147.

    Google Scholar 

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Authors and Affiliations

  1. Plant Cell and Tissue Culture Facility, Post-graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Orissa, 751004, India

    D. P. Barik, A. Mudgal & P. K. Chand

  2. Post-graduate Department of Biosciences and Biotechnology, Fakir Mohan University, Vyasa Vihar, Balasore, Orissa, 756019, India

    S. K. Naik

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  1. D. P. Barik
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  2. S. K. Naik
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  3. A. Mudgal
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  4. P. K. Chand
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Correspondence to P. K. Chand.

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Editor: A. Mentaberry

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Barik, D.P., Naik, S.K., Mudgal, A. et al. Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume. In Vitro Cell.Dev.Biol.-Plant 43, 144–148 (2007). https://doi.org/10.1007/s11627-007-9040-y

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