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Callus formation and plant regeneration from tubercles of Coryphantha elephantidens (Lem.) Lem.

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Summary

Callus cultures were established from pith tissue of Coryphantha elephantidens (Lem.) Lem. on Murashige and Skoog (MS) basal medium supplemented with 9.05 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.3 μM kinetin. Highest shoot regeneration frequency was observed on a medium containing 6.9 μM kinetin and 2.3 μM 2,4-D under 30 μE m−2 s−1 light intensity with a 16-h photoperiod. Calluses retained organogenic potential throughout several passages of subculture (18 mo.). Shoots were rooted on MS medium without plant growth regulators. All (100%) plantlets transplanted to soil survived acclimatization. Regenerated plants showed good overall growth and were morphologically similar to the mother plants.

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Author notes

  1. Brijmohan S. Bhau

    Present address: Laboratory of Cellular & Molecular Cytogenetics, Department of Botany, University of Delhi, 110007, India

Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, 180 006, Jammu, India

    A. K. Wakhlu & Brijmohan S. Bhau

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  1. A. K. Wakhlu
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  2. Brijmohan S. Bhau
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Wakhlu, A.K., Bhau, B.S. Callus formation and plant regeneration from tubercles of Coryphantha elephantidens (Lem.) Lem.. In Vitro Cell.Dev.Biol.-Plant 36, 211–214 (2000). https://doi.org/10.1007/s11627-000-0039-x

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  • DOI: https://doi.org/10.1007/s11627-000-0039-x

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