Skip to main content
SpringerLink
Log in

Genotypic and media effects on plant regeneration from cotyledon explant cultures of someBrassica species

  • General Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Conditions were established for efficient plant regeneration from cotyledon explant calli in different cultivars ofBrassica juncea, B. campestris andB. carinata on Murashige & Skoog's (MS) medium supplemented with various combinations of cytokinins and auxins. Regeneration frequency, however, varied with genotype and the different growth hormone combinations in media. Almost in all species, MS medium with zeatin (1.0 mg 1-1) and IAA (0.1 mg l-1) was found to be best for shoot organogenesis followed by the ones containing high kinetin (2.0 mg l-1) and low IAA (0.02 or 0.2 mg l-1) concentrations. On these media, the cotyledonary explants invariably underwent callusing followed by multiple shoot formation, which could be separated and subcultured for further propagation. Number of shoots per cotyledon explant cultured varied from 0 to as many as 50. InB. juncea andB. campestris, the regeneration frequency declined sharply in the absence of auxin in medium. BAP in combination with NAA yielded no or a reduced number of shoots. Shoot organogenesis also declined with the reduction in photoperiod from continuous light to 16 hours. Shoots were easily rooted during prolonged incubation on the same medium and whole plants were transferred to pots in the greenhouse and grown to maturity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

6-benzylaminopurine

KIN:

kinetin

IAA:

indole-3-acetic acid

MS:

medium after Murashige & Skoog [8]

NAA:

α-napthaleneacetic acid

ZEA:

Zeatin

References

  1. Bornman, CH (1985) Regeneration in vitro of economically important crop plants in the Nordic countries. Hereditas (Suppl) 3: 7–13

    Google Scholar 

  2. Chuong, PV, Beversdorf, WD (1985) High frequency embryogenesis through isolated microspore culture inBrassica napus L. andB. carinata Braun. Plant Sci 39: 219–226

    Google Scholar 

  3. Chuong, PV, Pauls, KP, Beversdorf, WD (1987) Protoplast culture and plant regeneration fromBrassica carinata Braun. Plant Cell Rep 6: 67–69

    Google Scholar 

  4. Dietert, MF, Barron, Sa, Yoder, OC (1982) Effects of genotype on in vitro culture in the genusBrassica. Plant Sci Lett 26: 233–240

    Google Scholar 

  5. Dunwell, JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers ofNicotiana tabacum. Environ Exp Bot 16: 109–118

    Google Scholar 

  6. Dunwell, JM (1981) In vitro regeneration from excised leaf discs of threeBrassica species. J Exp Bot 32: 789–799

    Google Scholar 

  7. Fazekas, GA, Sedmach, PA, Palmer, MV (1986) Genetic and environmental effects on in vitro shoot regeneration from cotyledon explants ofBrassica juncea. Plant Cell Tissue Org Cult 6: 177–180

    Google Scholar 

  8. Fu, Y, Jia, Y (1985) Plant regeneration from mesophyll protoplast culture of cabbage (Brassica oleracea var. capitata) Theor Appl Genet 71: 495–499

    Google Scholar 

  9. George, L, Rao, PS (1980) In vitro regeneration of mustard plants (Brassica juncea var. Rai-5) on cotyledon explants from non-irradiated irradiated and mutagen-treated seed. Ann Bot 46: 107–112

    Google Scholar 

  10. Glimelius, K (1984) High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61: 38–44

    Google Scholar 

  11. Govil, S, Babbar, SB, Gupta, SC (1986) Plant regeneration from in vitro cultured anthers of black mustard (Brassica nigra Koch) Plant Breeding 97: 64–71

    Google Scholar 

  12. Keller, WA, Armstrong, KC (1977) Embryogenesis and plant regeneration inBrassica napus anther cultures. Can J Bot 55: 1383–1388

    Google Scholar 

  13. Keller, WA, Armstrong, KC (1979) Stimulation of embryogenesis and haploid production inBrassica campestris anther cultures by elevated temperatures. Theor Appl Genet 60: 167–214

    Google Scholar 

  14. Klimaszewska, K, Keller, WA (1985) High-frequency plant regeneration from thin-layer explants ofBrassica napus. Plant Cell Tissue Org Cult 4: 183–197

    Google Scholar 

  15. Larkin, PJ, Scowcroft, WR (1981) Somaclonal variation—a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60: 197–214

    Google Scholar 

  16. Li, LC, Kohlenbach, HW (1982) Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts ofBrassica napus (L.) Plant Cell Rep 1: 209–211

    Google Scholar 

  17. Lichter, R (1982) Induction of haploid plants from isolated pollen ofBrassica napus. Z. Pflanzenphysiol 105: 427–434

    Google Scholar 

  18. Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  19. Rogozinska, JH, Drozdowska, L (1980) Organogenesis and plant formation from cotyledon and callus cultures of rape. Acta Soc Bot Pol 48: 5–20

    Google Scholar 

  20. Sacristan, MD (1985) Selection for disease resistance inBrassica cultures. Hereditas (Suppl) 3: 57–63

    Google Scholar 

  21. Singh, S, Chandra, N (1984) Plant regeneration in callus and suspension cultures ofBrassica campestris cv. Yellow Sarson. Plant Cell Rep 3: 1–4

    Google Scholar 

  22. Singh, S, Pareek, LK, Chandra, N (1981) Inducing differentiation from cultured cotyledonary leaves of mustard (Brassica juncea var. Prakash) Curr Sci 50: 412–414

    Google Scholar 

  23. Stavarek, SJ, Rains, DW (1984) The development of tolerance to mineral stress. HortSci 19: 377–382

    Google Scholar 

  24. Thomas, E, Hoffmann, F, Potrykus, I, Wenzel, G (1976) Protoplast regeneration and stem embryogenesis of haploid androgenetic rape. Mol Gen Genet 145: 245–247

    Google Scholar 

  25. Thomas, E, Wenzel, G (1975) Embryogenesis from microspores ofBrassica napus. Z Pflanzenzücht 79: 77–81

    Google Scholar 

  26. Wenzel, G (1980) Recent progress in microspore culture of crop plants. In: Davies, DR Hopwood, DA (Eds.) The Plant Genome, pp. 185–196. John Innes Charity, Norwich

    Google Scholar 

  27. Wenzel, G, Foroughi-Wehr, B, Friedt, W, Köhler, F, Oo, T (1985) Cell and tissue culture as supplementary tool in plant breeding: exemplified in potato, oilseed rape and barley. Hereditas (Suppl) 3: 15–25

    Google Scholar 

  28. Zee, SY, Hui, LH (1977) In vitro plant regeneration from hypocotyl and cotyledons of chinese kale (Brassica alboglabra Bailey) Z Pflanzenphysiol 82: 440–445.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Pflanzenbau und Pflanzenzüchtung, Justus-Liebig-Universität Giessen, D-6300, Giessen, FRG

    R. K. Jain & W. Friedt

  2. Department of Genetics, Haryana Agricultural University, 125 004, Hisar, India

    J. B. Chowdhury & D. R. Sharma

Authors
  1. R. K. Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. B. Chowdhury
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. R. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. Friedt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jain, R.K., Chowdhury, J.B., Sharma, D.R. et al. Genotypic and media effects on plant regeneration from cotyledon explant cultures of someBrassica species. Plant Cell Tiss Organ Cult 14, 197–206 (1988). https://doi.org/10.1007/BF00043410

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00043410

Key words

Search

Navigation