Abstract
Two new Ophiocordyceps species from Thailand, which belong to Ophiocordyceps nutans species complex and attack adult and nymph of stink bugs, are described: Ophiocordyceps asiana and O. tessaratomidarum. Molecular phylogenetic analyses of ITS and a combined sequence data of LSU, RPB1 and TEF1 revealed that these two new species are distinct from other known species in Ophiocordyceps nutans species complex. Based on the findings on morphological studies, they further support the naming of these two novel species. Ophiocordyceps asiana is parasitic on adult and nymph stink bugs, members of Coreidae and Pentatomidae which were recorded from China, Japan and Thailand, while O. tessaratomidarum is reported from Thailand for the first time and is only found as a parasite on adult stink bug, Tessaratoma papillosa (Tessaratomidae, Hemiptera). The fertile heads of O. asiana are shorter than reported for O. tessaratomidarum, and the colours of fertile heads producing teleomorph of these two species differs from each other by having strong reddish orange or light yellowish pink in O. asiana and pale yellow in O. tessaratomidarum.
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Data availability
All sequence data generated in this study (see Table 1) are available in GenBank (https://www.ncbi.nlm.nih.gov/genbank/).
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Acknowledgements
We are indebted to the Department of National Parks, Wildlife and Plant Conservation for their cooperation and support of our research project. We are also grateful to Heads of Phukhiao Wildlife Sanctuary, Phu Wiang National Park and Khao Yai National Park, and Mr. Sangaon Veeraping, the village headman of Ban Phaothai Community Forest, Phitsanulok province. We would like to thank the two anonymous reviewers and editors whose suggestions and comments helped to improve the manuscript.
Funding
This research was supported by the Platform Technology Management Section, National Center for Genetic Engineering and Biotechnology (BIOTEC), Grant No. P19-50231, the Research Personnel Development Division Central Office (NSTDA). Young Scientist and Technologist Program (YSTP) provided scholarships for W. Pooissarakul and S. Khao-ngam (Grant No. SCA-CO-2559-2421-TH and SCA-CO-2560-4637-TH, respectively).
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The specimens in this study were collected by S. Mongkolsamrit, S. Khao-ngam, W. Pooissarakul, W. Noisripoom, W. Himaman and J. J. Luangsa-ard. Morphological data were collected by S. Khao-ngam, W. Pooissarakul and S. Mongkolsamrit. Molecular data and phylogenetic analyses were performed by W. Noisripoom, S. Khao-ngam and W. Pooissarakul. Stink bugs were identified by J. Duangthisan. The project was supervised by J. J. Luangsa-ard. The original draft was written by S. Mongkolsamrit and N. Rungjindamai and it was reviewed and edited by J. J. Luangsa-ard. All authors commented and discussed on previous versions of the manuscript. All authors approved the final manuscript and agreed its publication in Mycological Progress.
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Khao-ngam, S., Mongkolsamrit, S., Rungjindamai, N. et al. Ophiocordyceps asiana and Ophiocordyceps tessaratomidarum (Ophiocordycipitaceae, Hypocreales), two new species on stink bugs from Thailand. Mycol Progress 20, 341–353 (2021). https://doi.org/10.1007/s11557-021-01684-x
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DOI: https://doi.org/10.1007/s11557-021-01684-x