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Arthrinium phaeospermum isolated from Tuber borchii ascomata: the first evidence for a “Mycorrhization Helper Fungus”?

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Abstract

A filamentous fungus was isolated from Tuber borchii Vitt. fruiting bodies, and it was identified as an Arthrinium phaeospermum (Corda) M.B. Ellis strain, an “endophyte” that forms various associations with healthy leaves, stems, and roots of plants. Molecular analysis confirmed the association of this filamentous fungus with the ascocarps of all collection sites in Salento, Apulia (South Italy). An in vitro symbiosis system between Cistus creticus L. and T. borchii was set up; A. phaeospermum appears to be able to promote mycorrhiza formation in Cistus seedlings, inducing primary root shortening and an increase of secondary roots, similar to the effect of Mycorrhization Helper Bacteria (MHB). Compartmented and uncompartmented bioassays were carried out to investigate the effects of exudates/volatiles released by the truffle-hosted fungus on root architecture; the results showed root shortening in compartmented bioassay suggesting that volatiles released by the fungus alone are sufficient to alter root morphology in early phase of interaction before the mycorrhiza formation. The first evidence for an influence of a truffle-hosted fungus on ectomycorrhizal symbiosis establishment is reported.

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Acknowledgments

Authors thank the Ministry of Education, Universities and Research (MIUR) that provides financial support for the Zeiss LSM 700 laser scanning microscope purchase (Enhancement Project 2HE, Cod. PONa3_00334—CUP F81D11000210007, Project of Structural Enhancement financed by PON R&C 2007—2013, Axis I Operative 4.1.1.4, Action I “Structural Improvement”).

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Authors and Affiliations

  1. Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce – Monteroni, 73100, Lecce, Italy

    Erika Sabella, Eliana Nutricati, Alessio Aprile, Antonio Miceli & Luigi De Bellis

  2. Department of Biology, University of Pisa, via Luca Ghini 13, 56126, Pisa, Italy

    Carlo Sorce & Roberto Lorenzi

Authors
  1. Erika Sabella
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  2. Eliana Nutricati
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  3. Alessio Aprile
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  4. Antonio Miceli
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  5. Carlo Sorce
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  6. Roberto Lorenzi
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  7. Luigi De Bellis
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Correspondence to Erika Sabella.

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Sabella, E., Nutricati, E., Aprile, A. et al. Arthrinium phaeospermum isolated from Tuber borchii ascomata: the first evidence for a “Mycorrhization Helper Fungus”?. Mycol Progress 14, 59 (2015). https://doi.org/10.1007/s11557-015-1083-6

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  • DOI: https://doi.org/10.1007/s11557-015-1083-6

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