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Single-cell genomics: An overview

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Frontiers in Biology

Abstract

The newly developed next-generation sequencing platforms, in combination with genome-scale amplification methods, provide a powerful tool to study genomics from a single cell. This mini-review summarizes the technologies of single cell genomics and their applications in several areas of biomedical research including stem cells, cancer biology and reproductive medicine. Particularly, it highlights recent advances in single cell exome sequencing, RNA-seq, and genome sequencing. The application of these powerful techniques will shed new light on the fundamental principles of gene transcription and genome organization at single-cell level and improve our understanding of cellular heterogeneity and diversity in multicellular organisms.

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Authors and Affiliations

  1. Stem Cell Research Center, Department of Regenerative Medicine, Tongji University School of Medicine, Tongji University, Shanghai, 200092, China

    Qichao Wang, Xianmin Zhu, Yun Feng & Zhigang Xue

  2. Advanced Institute of Translational Medicine, Tongji University, Shanghai, 200092, China

    Xianmin Zhu & Guoping Fan

  3. School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China

    Guoping Fan

  4. Department of Human Genetics, UCLA, Los Angeles, CA, 90095, USA

    Guoping Fan

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  1. Qichao Wang
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  2. Xianmin Zhu
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  3. Yun Feng
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  4. Zhigang Xue
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  5. Guoping Fan
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Correspondence to Guoping Fan.

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Wang, Q., Zhu, X., Feng, Y. et al. Single-cell genomics: An overview. Front. Biol. 8, 569–576 (2013). https://doi.org/10.1007/s11515-013-1285-8

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