Abstract
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed. Specific immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/S. cho in rabbits. Results showed that T cell response and specific antibody production were elicited. This approach may present a general strategy for eliciting immune responses with DNA vaccine delivered by live bacterial vectors. The stimulated indexes of T lymphoproliferation by specific antigens of FMDV in rabbits, can reach up to 11.0 and an antibody titer of 1/32 as detected in the erum with liquid block ELISA.
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References
Bachrach H.-L., Foot-and-mouth disease virus, Annu. Rev. Microbiol., 1968, 22: 201–244
Curtiss R.-T., Hassan J.-O., Herr J., Kelly S.-M., Levine M., Mahairas G.-G., Milich D., Peterson D., Schodel F., Srinivasan J., Tacker C., Tinge S.-A., Wright R., Nonrecombinant and recombinant avirulent salmonella vaccine. Talwar G.-P., Rao K.-V.-S, Chauhan V-S. Recombinant and Synthetic Vaccines, New Delhi India: Norosa Publishing House, 1994, 340–351
Darji A., Guzman C.-A., Gerstel B., Wachholz P, Timmis K.-N, Wehland J. Chakraborty T, Weiss S. Oral somatic transgene vaccination using attenuated S. typhimurium, Cell, 1997, 91: 765–775
Darji A., Lage S.-Z., Garbe A.-I., Chakraborty T., Weiss S., Oral delivery of DNA vaccines using attenuated Salmonella typhimurium as carrier, FEMS Immunol. Med. Microbiol., 2000, 27(4): 341–349
Dietrich G., Gentschev I., Hess J., Ulmer J.-B., Kaufmann S.-H.-E., Goebel W., Delivery of DNA vaccines by attenuated intracellular bacteria, Trends Immunol. Today, 1999, 20(6): 251–253
Fagan P.-K., Djordjevic S.-P., Chin J., Eamens G.-J., Walker M.-J., Oral immunization of mice with attenuated salmonella typhimurium aroA expressing a recombinant mycoplasma hyopneumoniae antigen (NrdF), Infect and Immun., 1997, 65: 2502–2507
Fang X.-W., Li Y.-L., Huang C.-B., Zheng M., Feng W.-D., Sun W., Live vaccine against swine paratyphoid from the attenuated smooth strain C500 of Salmonella choleraesuis. Acta Veterinaria et Zootechnica Sinica. 1981, 12(2): 99–106
Hess J., Gentschev I., Miko D., Welzal M., Ladel C., Gvebel W., Kaufmann S.-H.-E., Superior efficacy of secreted over somatic antigen display in recombinant Salmonella vaccine induced protection against listeriosis, Proc. Natl. Acad. Sci. USA, 1996, 93: 1458–1463
Huang C.-B., Feng W.-D., Xue M.-Q., Zhang X.-J., Yang S.-Z., Yang S.-J., Dou Y.-M., Jia S.-G., Oral administration of the live vaccine against swine paratyphoid. Scientia Agricultura Sinica. 1981, 6: 89–94.
Huang X.-B., Yang Z.-J., Xu Q.-X, Sheng Z.-T, Xie Y, Yan W.-Y, You Y.-J, Sun L.-C., Zheng Z.-X., Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine, Virol. Immunol., 1999, 12(1): 1–8
Joseph S., David W.-R., Laboratory Manual of Molecular Cloning, Beijing: Science Publish, 1998
Neutra M.-R., M cells in antigen sampling in mucosal tissues, Curr. Top Microbiol. Immunol., 1999, 236: 17–32
Paglia P., Medina E., Arioli I., Guzman C.-A, Colombo M.-P., Gene transfer in dendritic cells induced by oral DNA vaccination with salmonella typhimurium results in protective immunity against a murine fibrosarcoma, Blood, 1998, 92(9): 3172–3176
Shata M.-I., Stevceva L., Agwale S, Lewis G.-K, Hone D.-M., Recent advances with recombinant bacterial vaccine vectors, Mol. Med. Today, 2000, 6: 66–71
Shiau A.-L., Chen Y.-L, Liao C.-Y., Huang Y.-S., Wu C.-L., Prothymosin enhances protective immune responses induced by oral DNA vaccination against pseudorabies delivered by Salmonella choleraesuis, Vaccine, 2001, 19: 3947–3956
Susan K., H., Stocker B.-A.-D., Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines, Nature, 1981, 291: 238–239
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Translated From Journal of Fudan University(Natural Science), 2005,44(4)[译自: 复旦学报(自然科学版), 2005,44(4)]
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Liu, M., Niu, X., Yan, J. et al. Immure response induced by oral DNA vaccination against FMDV delivered by attenuated Salm onella choleraesuis C500. Front. Biol. China 1, 110–114 (2006). https://doi.org/10.1007/s11515-006-0020-0
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DOI: https://doi.org/10.1007/s11515-006-0020-0