We studied immunogenicity of two recombinant proteins FR.9 and FR.11-3 created on the basis of fragments of the primary structure of N. meningitidis IgA1 protease with different molecular weights containing different sets of T and B epitopes. The proteins actively protect animals infected with live virulent culture of meningococci, serogroups A, B, and C. Analysis of CD4+, CD8+, and CD19+ lymphocyte populations in mouse blood showed predominant contribution of different cell populations to the formation of immune response to different proteins. Injection of FR.11-3 protein to animals did no affect the immunoregulatory index, hence, this protein can be used for creation of immunologically safe vaccine preparation.
Article PDF
Similar content being viewed by others
References
Alliluev AP, Kotelnikova OV, Zinchenko AA, Prokopenko YuA, Zhigis LS, Razgulyaeva OA, Melikhova TD, Nokel EA, Drozhzhina EYu, Rumsh LD. Potential Polyvaccine Based on Microbial IgA1 Protease for Prophylaxis of Bacterial Meningitis. Epidemiol. Vaktsinoprof. 2016;15(6):88-94. Russian.
Kotel’nikova OV, Alliluev AP, Drozhzhina EYu, Koroleva IS, Sitnikova EA, Zinchenko AA, Gordeeva EA, Melikhova TD, Nokel EA, Zhigis LS, Zueva VS, Razgulyaeva OA, Serova OV, Yagudaeva EYu, Rumsh LD. Protective properties of recombinant igA1 protease from meningococcus. Biomed. Khim. 2014;60(4):479-486. Russian.
The Use of Flow Cytometry for Evaluation of Functional Activity of the Human Immune System. Moscow, 2001. Russian.
Serova OV, Melnikov EE, Zinchenko AA, Kotel’nikova OV, Gordeeva EA, Zhigis LS, Zueva VS, Melikhova TD, Nokel EA, Yagudaeva EY, Rumsh LD, Bichucher AM, Kozlov LV, Alliluev AP. Recombinant IGA1 protease from N. meningitidis. Obtaining and properties. Biofarm. Zh. 2011;3(6):42-47. Russian.
Yarilin AA. Natural regulatory T cells. Ross. Med. Zh. 2007; (1):43-48. Russian.
Yarilin AA, Donetskova AD. The natural regulator T-cells and FOXP3 factor. Immunologiya. 2006;27(3):176-188. Russian.
AlonsoDeVelasco E, Verheul AF, Verhoef J, Snippe H. Streptococcus pneumoniae: virulence factors, pathogenesis, and vaccines. Microbiol. Rev. 1995;59(4):591-603.
Crum-Cianflone N, Sullivan E. Meningococcal Vaccinations. Infect. Dis. Ther. 2016;5(2):89-112.
Gupta SK, Smita S, Sarangi AN, Srivastava M, Akhoon BA, Rahman Q, Gupta S.K. In silico CD4+ T-cell epitope prediction and HLA distribution analysis for the potential proteins of Neisseria meningitidis Serogroup B--a clue for vaccine development. Vaccine. 2010;28(43):7092-7097.
Kirkeby L, Rasmussen TT, Reinholdt J, Kilian M. Immunoglobulins in nasal secretions of healthy humans: structural integrity of secretory immunoglobulin A1 (IgA1) and occurrence of neutralizing antibodies to IgA1 proteases of nasal bacteria. Clin. Diagn. Lab. Immunol. 2000;7(1):31-39.
Tsirpouchtsidis A, Hurwitz R, Brinkmann V, Meyer TF, Haas G. Neisserial immunoglobulin A1 protease induces specific T-cell responses in humans. Infect. Immun. 2002;70(1):335-344.
Zinchenko AA, Alliluev AP, Serova OP, Gordeeva EA, Zhigis LS, Zueva VS, Razgulyaeva OA, Melikhova TD, Nokel EA, Drozhzhina EYu, Kotelnikova OV, Rumsh LD. Immunogenic and Protective Properties of Recombinant Proteins Based on Meningococcal IgA1 Protease. J. Meningitis. 2015;1(1). doi: https://doi.org/10.4172/2572-2050.1000102.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 6, pp. 722-725, June, 2018
Rights and permissions
About this article
Cite this article
Kotel’nikova, O.V., Alliluev, A.P., Zinchenko, A.A. et al. Peculiarities of the Formation of Antimeningococcus Immunity in Mice Immunized with Fragments of N. meningitidis IgA1 Protease. Bull Exp Biol Med 165, 763–766 (2018). https://doi.org/10.1007/s10517-018-4260-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4260-1