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Spatial distribution of bacterial community in EGSB reactor treating synthetic sulfate-containing wastewater at low organic loading rate

  • Article
  • Environmental Chemistry
  • Published:
Chinese Science Bulletin

Abstract

Given that the consumption of organic substances entails costly biodesulfurization, the characteristics of the bacterial community in a reactor should be determined to increase the desulfurizing rate under low organic loading condition. In this study, the bacterial community distribution in the expanded granular sludge bed reactor used to treat sulfate-containing wastewater with low organic loading rate was determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rDNA clone library analyses. DGGE results showed that the predominant bacteria were stable and accounted for ~90 % sulfate removal efficiency. Differences in band positions and intensities indicated that the distribution and abundance of bacteria were affected by their positions in the reactor. Typical bands were identified in the bacterial community comprising Desulfovibrio, Desulfomicrobium, Thiomonas, Acinetobacter, Bacteroidetes, and Chloroflexi. Their functions in the reactor were also discussed. The possible links between the functional and microbial responses were also investigated based on the characteristic and spatial distribution of each bacterium in consortium.

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Acknowledgments

This work was supported by The National High Technology Research and Development Program of China (2011AA060702, 2011AA060904) and the Open Funding Project of the National Key Laboratory of Biochemical Engineering (2012KF-07).

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China

    Kai Zhang, Xiaolei Cao, Yuxing Sheng & Hongbin Cao

  2. National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Beijing, 100190, China

    Kai Zhang, Xiaolei Cao, Yuxing Sheng & Hongbin Cao

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Xiaolei Cao

Authors
  1. Kai Zhang
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  2. Xiaolei Cao
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  3. Yuxing Sheng
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  4. Hongbin Cao
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Corresponding authors

Correspondence to Yuxing Sheng or Hongbin Cao.

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Zhang, K., Cao, X., Sheng, Y. et al. Spatial distribution of bacterial community in EGSB reactor treating synthetic sulfate-containing wastewater at low organic loading rate. Chin. Sci. Bull. 59, 54–61 (2014). https://doi.org/10.1007/s11434-013-0043-2

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  • DOI: https://doi.org/10.1007/s11434-013-0043-2

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