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Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora

  • Articles/Microbiology
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Chinese Science Bulletin

Abstract

Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Seawater culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4–10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose consumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    JinLing Cai & GuangCe Wang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    JinLing Cai

  3. Key Laboratory of Marine Resources and Chemistry of Tianjin, Research Center for Algal Bioenergy and Environmental Effect, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China

    GuangCe Wang, DaLing Zhu & GuangHua Pan

  4. Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650224, China

    YanChuan Li

Authors
  1. JinLing Cai
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  2. GuangCe Wang
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  3. YanChuan Li
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  4. DaLing Zhu
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  5. GuangHua Pan
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Corresponding author

Correspondence to GuangCe Wang.

Additional information

Supported by the National High Technology Research and Development Program of China (Grant No. 2006AA05Z112)

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Cai, J., Wang, G., Li, Y. et al. Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora. Chin. Sci. Bull. 54, 2656–2661 (2009). https://doi.org/10.1007/s11434-009-0399-5

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  • DOI: https://doi.org/10.1007/s11434-009-0399-5

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