Skip to main content
SpringerLink
Log in

Development of Triticum aestivum-Leymus racemosus ditelosomic substitution line 7Lr#1S(7A) with resistance to wheat scab and its meiotic behavior analysis

  • Articles/Crop Genetics
  • Published:
Chinese Science Bulletin

Abstract

Leymus racemosus is highly resistant to wheat scab (Fusarum head bright). The transfer of scab resistant gene from L. racemosus to Triticum aestivum is of great significance for broadening the base of wheat resistance. In the present study, the pollen of T. aestivum-L. racemosus monosomic addition line with scab resistance was treated by irradiation with 1200 R 60Co-γ-rays prior to pollinating to emasculated wheat cv. Mianyang 85–45. Nine plants with a telocentric chromosome 7Lr#1S were observed in M1, and one ditelosomic substitution line 7Lr#1S was selected from selfcrossing progenies and confirmed by chromosome C-banding and GISH. Furthermore, a co-dominant EST-SSR marker CINAU 31 was employed to identify this substitution line. A pair of chromosome 7A of common wheat were found to be replaced by a pair of telocentric chromosome 7Lr#1S, and further investigation showed that chromosome configuration of the substitution line at MI of PMCs after GISH was 17.50

W + 2.19

W + 0.42

7Lr#1S + 1.08 I7Lr#1S + 0.69 IW. Two telocentric chromosomes paired as a bivalent in 59.7% of PMCs. Abnormal chromosome behaviors of telocentric chromosomes were observed in part of PMCs at anaphase I and telophase I, including the moving of two telocentric chromosomes to the same pole, lagging and earlier separation of their sister chromatid. All these abnormal behaviors can be grouped into three distinct types of tetrads according to different numbers of 7Lr#1S in their daughter cells and various micronucleus in some tetrads. However, due to the high transmission frequency of the female and male gametes with a 7Lr#1S, 84% of the selfcrossing progeny plants had ditelosomic substitution. The substitution line showed high resistance to wheat scab in a successive two-year test both in the greenhouse and field; hence, the line will be particularly valuable for alien gene mapping, small fragment translocation induction and telosomic cytological behavior analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. McGuire P E, Dvorak J. High salt-tolerance potential in wheatgrasses. Crop Sci, 1981, 21: 702–705

    Google Scholar 

  2. Mujeeb-Kazi A, Bekele G T, Mirand J L. Incorporation of alien genetic information from Elymus giganteus into Triticum aestivum. In: Proc the 6th Int Wheat Genet Symp, Kyoto, Japan, 1983. 223–231

  3. Chen P D, Wang Z T, Wang S L, et al. Transfer of useful germplasm from Leymus racemosus Lam. to common wheat. III. Development of addition lines with wheat scab resistance (in Chinese). Acta Genet Sin, 1995, 22: 206–210

    Google Scholar 

  4. Qi L L, Wang S L, Chen P D. Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat. Theor Appl Genet, 1997, 95: 1084–1091

    Article  CAS  Google Scholar 

  5. Sun W X, Chen P D, Liu D J. Transfer of useful germplasm from Leymus racemous Lam. to common wheat, VI. Development and identification of T. aestivum-L. racemosus telosomic addition lines (in Chinese). Acta Genet Sinica, 1998, 25(3): 259–264

    CAS  Google Scholar 

  6. Kishii M, Yamada T, Sasakuma T. Production of wheat-Leymus racemosus chromosome addition lines. Theor Appl Genet, 2004, 109: 255–260

    Article  PubMed  CAS  Google Scholar 

  7. Gill B S, Friebe B, Endo T R. Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome, 1991, 34: 830–839

    Google Scholar 

  8. Chen P D, Qi L L, Zhou B. Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet, 1995, 91: 1125–1128

    Article  Google Scholar 

  9. Zhang P, Li W, Friebe B. Simultaneous painting of three genomes in hexploid wheat by BAC-FISH. Genome, 2004, 47: 979–987

    Article  PubMed  CAS  Google Scholar 

  10. Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res, 1991, 19: 1349

    Article  PubMed  CAS  Google Scholar 

  11. Chen H M, Li L Z, Wei X Y. Development, chromosome location and genetic mapping of EST-SSR markers in wheat. Chin Sci Bull, 2005, 50(20): 2328–2336

    Article  CAS  Google Scholar 

  12. Chen J F, Ren Z L, Gao L F. Developing new SSR markers from EST of wheat. Acta Agron Sin, 2005, 31(2): 154–158

    Google Scholar 

  13. Sears E R. The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 1956, 9: 1–22

    Google Scholar 

  14. Driscoll C J, Jensen N F. Characteristics of leaf rust resistance transferred from rye to wheat. Crop Sci, 1964, 4: 372–374

    Google Scholar 

  15. Driscoll C J, Jensen N F. Release of a wheat-rye translocation stock involving leaf rust and powdery mildew resistance. Crop Sci, 1965, 5: 279–280

    Google Scholar 

  16. Sharma D, Knott D R. The transfer of leaf-rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol, 1966, 8: 137–143

    Google Scholar 

  17. Driscoll C J, Anderson L M. Cytogenetic studies of transec-a wheat-rye translocation line. Can J Genet Cytol, 1967, 9: 375–380

    Google Scholar 

  18. Mukai Y, Friebe B, Hatchett J H. Molecular cytogenetic analysis of radiation-induced wheat-rye terminal and intercalary chromosomal translocations and the detection of rye chromatin specifying resistance to Hessian. Chromosoma, 1993, 102: 88–95

    Article  Google Scholar 

  19. Sears E R. Use of radiation to transfer alien chromosome segments to wheat. Crop Sci, 1993, 33: 897–901

    Google Scholar 

  20. Bie T D, Cao Y P, Chen P D. Mass Production of intergeneric chromosomal translocations through pollen irradiation of Triticum durum-Haynaldia villosa amphiploid. J Integrat Plant Biol, 2007, 49(11): 1619–1626

    Article  Google Scholar 

  21. Pienaar R de V, van Niekerk H A. The effect of gamma-irradition on wheat gametophytes. In: Proc the 4th Int Wheat Genet Symp. Columbia, Missouri, USA, 1973. 289–294

  22. Sears E R. The aneuploids of common wheat. Mo Agr Exp Stn Res Bull, 1954, 572: 1–58

    Google Scholar 

  23. Sears E R. Chromosome mapping with the aid of telocentrics. In: Proc the 2nd Int Wheat Genet Symp. Stockholm, 1966. 370–381

  24. Sears E R, Sears Lotti M S. The telocentric chromosomes of common wheat. In: Proc the 4th Int Wheat Genet Symp. New Delhi, 1978. 389–407

  25. Sears E R. Transfer of alien genetic material to wheat. In: Evans LT, Peacock W J, eds. Wheat Science-Today and Tomorrow. Cambridge: Cambridge Univerisity Press, 1981. 75–89

    Google Scholar 

  26. Gupta P K, Rustgi S, Sharma S. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Mol Gen Genom, 2003, 270: 315–323

    Article  CAS  Google Scholar 

  27. Gao L F, Jing R L, Huo N X. One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat. Theor Appl Genet, 2004, 108: 1392–1400

    Article  PubMed  CAS  Google Scholar 

  28. Ge R C, Zhao M L, Zhao B C. Study on the C banding of Leymus multicaulis, Puna chicory root tip chromosomes by HBSG method. J Hebei Normal Univ (Nat Sci Ed), 2006(30), 2: 213–216

    Google Scholar 

  29. Burnham C R, John T, Stout W. Chromosome pairing in maize. Genetics, 1972. 71: 111–126

    PubMed  Google Scholar 

  30. Ding D Q, Yamamoto A, Haraguchi T. Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast. Dev Cell, 2004, 6: 329–34

    Article  PubMed  CAS  Google Scholar 

  31. Zhao M L, Li R F, Liang H X. The behavior of an alien chromosome in Triticum aestivum-Leymus multicaulis monosomic addition lines during meiosis. Prog Nat Sci, 2001, 11: 945–949

    Google Scholar 

  32. Li R F, Liang H X, Zhao M L. The meiotic behavior of an alien chromosome in Triticum aestivum-Haynaldia villosa monosomic addition lines. Scient Agricult Sin, 2002, 35(2): 127–131

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, China

    LinSheng Wang & PeiDu Chen

  2. College of Agronomy, Henan University of Science and Technology, Luoyang, 471003, China

    LinSheng Wang

Authors
  1. LinSheng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. PeiDu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to PeiDu Chen.

Additional information

Supported by the National Hi-Tech Research and Development Program of China (Grant No. 2006AA10Z1F6), National Natural Science Foundation of Jiangsu Province (Grant No. BK2006720), High Tech Research Plan of Jiangsu Province (Grant No. BG2005310) and Program of Introducing Talents of Discipline to Universities (Grant No. B08025)

About this article

Cite this article

Wang, L., Chen, P. Development of Triticum aestivum-Leymus racemosus ditelosomic substitution line 7Lr#1S(7A) with resistance to wheat scab and its meiotic behavior analysis. Chin. Sci. Bull. 53, 3522–3529 (2008). https://doi.org/10.1007/s11434-008-0457-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-008-0457-4

Keywords

Search

Navigation