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Construction of mini-Tn4001 transposon vector for Mycoplasma gallisepticum

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Abstract

The detailed genetic analysis of mycoplasmas has long been hampered by the lack of appropriate tools for genetic manipulation. In this study, the transposon vector, mini-Tn4001tetM, was constructed containing the tnp gene, encoding a transposase gene in Staphylococcus aureus, two copies of the IS256 inverted repeat sequence (inner and outer) and the tetM gene, from the Enterococcus faecalis Tn916 transposon, conferring resistance to tetracycline. This vector was electro-transformed into Mycoplasma gallisepticum (MG). The recombinant cells were screened by tetracycline selection. The results indicated that the transposon vector could replicate in MG strain R by successive passages, indicating that MG is a potential vector for expressing protective antigens of other pathogens.

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China

    HongJun Chen, ChunMei Zhao, XieYue Shen, DanQing Chen, ShengQing Yu & Chan Ding

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  1. HongJun Chen
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  2. ChunMei Zhao
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  3. XieYue Shen
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  4. DanQing Chen
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  5. ShengQing Yu
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  6. Chan Ding
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Correspondence to Chan Ding.

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Chen, H., Zhao, C., Shen, X. et al. Construction of mini-Tn4001 transposon vector for Mycoplasma gallisepticum. Sci. China Life Sci. 53, 1340–1345 (2010). https://doi.org/10.1007/s11427-010-4082-5

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  • DOI: https://doi.org/10.1007/s11427-010-4082-5

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