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Alkylphthalides with intracellular triglyceride metabolism-promoting activity from the rhizomes of Cnidium officinale Makino

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Abstract

Methanol extract of the Cnidium officinale Makino rhizome, which is used as a crude drug Cnidium Rhizome (Cnidii Rhizoma; “Senkyu” in Japanese) and is listed in the Japanese Pharmacopoeia XVIII, showed intracellular triglyceride metabolism-promoting activity in high glucose-pretreated HepG2 cells. Thirty-five constituents, including two new alkylphthalide glycosides, senkyunosides A (1) and B (2), and a neolignan with a new stereoisomeric structure (3), were isolated in the extract. Their stereostructures were elucidated based on chemical and spectroscopic evidence. Among the isolates, several alkylphthalides, (Z)-3-butylidene-7-methoxyphthalide (9) and senkyunolides G (10), H (14), and I (15), and a polyacetylene falcarindiol (26), were found to show significant activity without any cytotoxicity at 10 μM.

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Acknowledgements

The authors wish to thank the Division of Joint Research Center, Kindai University for the NMR and MS measurements, and Editage (www.editage.com) for English language editing.

Funding

This work was supported by the JSPS KAKENHI, Japan [Grant Number: 22K06688 (T.M.)].

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Author notes

  1. Naoki Inoue

    Present address: School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University, 11-68 Koshien Kyuban-cho, Nishinomiya, Hyogo, 663-8179, Japan

  2. Kiyofumi Ninomiya

    Present address: School of Pharmacy, Shujitsu University, 1-6-1 Nishigawara, Naka-Ku, Okayama, 703-8516, Japan

Authors and Affiliations

  1. Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Toshio Morikawa, Naoki Inoue, Saya Yamamoto, Miyuki Shiotani, Yoshiaki Manse & Kiyofumi Ninomiya

  2. Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Toshio Morikawa

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Conceptualization, T.M.; methodology, T.M. and K.N.; investigation, N.I., S.Y., M.S., and Y.M.; writing, review, and editing, T.M. All authors have read and approved the published version of the manuscript.

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Correspondence to Toshio Morikawa.

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Morikawa, T., Inoue, N., Yamamoto, S. et al. Alkylphthalides with intracellular triglyceride metabolism-promoting activity from the rhizomes of Cnidium officinale Makino. J Nat Med (2024). https://doi.org/10.1007/s11418-024-01799-x

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