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Manure fertilization enhanced microbial immigration in the wheat rhizosphere

  • Soils, Sec 5 • Soil and Landscape Ecology • Research Article
  • Published:
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Abstract

Purpose

Microbial immigration is essential for understanding microbial mechanisms involved in nutrient cycling under different fertilization strategies. Here, we aimed to illustrate the effects of fertilization on microbial immigration in the wheat rhizosphere.

Materials and methods

Bacterial 16S rRNA gene and fungal ITS sequencing were applied to determine the microbial composition in the wheat rhizosphere. Co-occurrence networks, spatiotemporal source models, and structural equation models (SEMs) were conducted to investigate microbial immigration in two different niches (bulk soil and rhizosphere) and at two growth stages with different fertilization strategies (control without fertilization (CK), chemical fertilization (NPK), and organic manure fertilization (OM)).

Results and discussion

OM greatly decreased the network complexity ratio of the rhizosphere to bulk soil for bacteria (CK/NPK/OM ratio: 4.14/1.65/0.64) and fungi (CK/NPK/OM ratio: 1.09/1.35/0.72). The proportion of bacterial and fungal communities in the rhizosphere sourcing from the bulk soil was highest under OM treatment (94.34%; 88.78%), followed by NPK (88.69%; 83.86%) and CK (85.30%; 76.40%) treatments. The jointing stage contributed more than the flowering stage to microbial immigration. In comparison to fungi, the proportion of bacteria in the rhizosphere derived from the bulk soil was much higher. Structural equation model indicated that the increase of total carbon under OM treatment affected the composition of bacteria and fungi by promoting microbial immigration from the bulk soil to the rhizosphere.

Conclusions

OM treatment enhanced microbial immigration in the wheat rhizosphere, providing novel insights into plant-soil-microbiota interactions in the wheat rhizosphere.

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Funding

This study was financially supported by the National Key Research and Development Program of China (2021YFD1900100), the Project for the China Agricultural University (2020TC050 & 2020TC144), and the Program of Advanced Discipline Construction in Beijing, and 2115 Talent Development Program of China Agricultural University.

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, Key Laboratory of Plant-Soil Interaction, Ministry of Education, National Academy of Agriculture Green Development, China Agricultural University, Beijing, 100193, China

    Ye Liu, Shuikuan Bei, Oladele Olatunde, Ying Li, Xingjie Wu, Hongyan Zhang, Zhenling Cui & Jingjing Peng

  2. Institute of Environmental Microbiology, College of Resource and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Christopher Rensing

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  1. Ye Liu
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Correspondence to Jingjing Peng.

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Liu, Y., Bei, S., Olatunde, O. et al. Manure fertilization enhanced microbial immigration in the wheat rhizosphere. J Soils Sediments 22, 1828–1837 (2022). https://doi.org/10.1007/s11368-022-03185-0

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