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An efficient approach towards the bioremediation of copper, cobalt and nickel contaminated field samples

  • Soils, Sec 4 • Ecotoxicology • Research Article
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Abstract

Purpose

The synthetic soil based bioremediation approach as reasonable and sustainable practice at the farming level where desired bioremediation could be established at lower cost.

Materials and methods

Metal-tolerant bacteria from different environmental field samples, (a) a municipal dump site, (b) an agricultural field and (c) sludge of electro-plating industries, were screened and characterized. Bioremediation of metal contaminants through isolated bacteria was compared under two different conditions, synthetic soil and basic minimal media containing copper, cobalt and nickel.

Results and discussion

The pollutants arising from industrial effluents are imparting a huge negative impact on agricultural land. Microbes are predominant in heavy metal-contaminated sites, which signifies as a potential opportunity for the researchers towards bioremediation. Three bacterial species showed high metal tolerance; 16S ribosomal DNA (rDNA) analysis revealed that the organisms were Proteus vulgaris strain, Stenotrophomonas sp. and Bacillus thuringiensis. Percentage removal of metals was also analysed under different concentrations and pH.

Conclusions

The current tested methods are helpful in streamlining the natural compliance of fragile elements and its uptake into the microbial system under in vitro and in situ conditions.

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Acknowledgments

This research was supported by the Amity University Uttar Pradesh and Lovely Professional University, Punjab, India. The authors are grateful to the University for the support.

Author contributions

MK and JS both are having joint corresponding authorship based on the equal contribution. MK, JS, VK, AV, AP, AKS and AA were involved in manuscript designing and data interpretation. AP was involved in sample collection and analysis. JS and RP were involved in the statistical analysis. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, UP, 201 303, India

    Manoj Kumar, Vivek Kumar, Ajit Varma & Ram Prasad

  2. School of Biotechnology and Biosciences, Lovely Professional University, Phagwara, Punjab, 144 411, India

    Amarish Kumar Sharma, Abhradip Pal & Joginder Singh

  3. Defence Research & Development Organisation, Defence Institute of Bio-Energy Research (DIBER), Haldwani, Uttarakhand, 263 139, India

    Anfal Arshi

Authors
  1. Manoj Kumar
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  2. Vivek Kumar
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  3. Ajit Varma
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  4. Ram Prasad
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  5. Amarish Kumar Sharma
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  6. Abhradip Pal
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  7. Anfal Arshi
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  8. Joginder Singh
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Correspondence to Manoj Kumar or Joginder Singh.

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The authors declare that they have no conflict of interests.

Additional information

Responsible editor: Kirk T. Semple

Manoj Kumar and Vivek Kumar contributed equally to this article.

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Kumar, M., Kumar, V., Varma, A. et al. An efficient approach towards the bioremediation of copper, cobalt and nickel contaminated field samples. J Soils Sediments 16, 2118–2127 (2016). https://doi.org/10.1007/s11368-016-1398-1

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  • DOI: https://doi.org/10.1007/s11368-016-1398-1

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