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Archaeal communities in the sediments of three contrasting mangroves

  • SEDIMENTS, SEC 4 • SEDIMENT-ECOLOGY INTERACTIONS • RESEARCH ARTICLE
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Abstract

Purpose

This study evaluates the presence and diversity of 16S rRNA (rrs) and amoA genes from archaea in three mangrove sediments under different stages of preservation (one pristine mangrove, one affected by anthropogenic activity, and another contaminated by an oil spill) in the state of São Paulo (Brazil).

Materials and methods

A combination of DGGE, coupled with ordination analysis, and clone libraries of both targeted genes (rrs and amoA) was used to infer the diversity and phylogeny of archaeal communities in the mangrove analyzed samples.

Results and discussion

The DGGE combined with multivariate analysis revealed, based on the ribosomal gene, differences in archaeal communities according to environmental parameters such as mangrove location, anthropogenic activity, and oil contamination. The profiles based on the amoA gene were more similar than those obtained for the gene rrs, with the only difference statistically observed for the community found in the mangrove under anthropogenic pressure. Furthermore, phylogenetic analysis revealed most archaeal groups affiliated to sequences belonging to the Thaumarchaeota (53.1%, 24 OTUs) and Euryarchaeota (29.6%, 14 OTUs) phyla, in addition to 14 sequences affiliated to an unclassified Archaea (16.1%, 8 OTUs). Concerning the analysis of the amoA gene, mangroves harbored sequences affiliated with those previously described in water column and soil/sediment samples, besides two possible clusters specifically found in mangroves.

Conclusions

The findings are that the mangroves act as a reservoir for archaeal diversity, are possibly involved in nitrogen transformation in this ecosystem, and are affected by distinct pressures caused by anthropogenic activities.

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Acknowledgments

This study was supported by a grant from the State of São Paulo Research Foundation (FAPESP/BIOTA 2004/13910-6). F.D. Andreote received a postdoctoral fellowship from FAPESP (2007/56360-4), and R.G. Taketani was a recipient of a postdoctoral grant from CNPq. Also, A.C.F. Dias received a graduate fellowship (2008/54013-8). We also thank the support from the Oceanographic Institute (IO, USP, São Paulo), especially Dr. Ricardo P. Menghini and João L. Silva for their support in mangrove expeditions and samplings.

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Authors and Affiliations

  1. Laboratory of Environmental Microbiology, CNPMA — Embrapa Environment, Jaguariúna, Brazil

    Armando Cavalcante Franco Dias, Francisco Dini-Andreote & Itamar Soares de Melo

  2. Department of Genetics, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), University of São Paulo, Piracicaba, Brazil

    Francisco Dini-Andreote

  3. Center for Nuclear Energy in Agriculture, CENA/USP, University of São Paulo, Piracicaba, Brazil

    Armando Cavalcante Franco Dias, Rodrigo Gouvêa Taketani, Siu Mui Tsai & João Lúcio Azevedo

  4. Department of Soil Science, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), University of São Paulo, Piracicaba, Brazil

    Fernando Dini Andreote

Authors
  1. Armando Cavalcante Franco Dias
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  2. Francisco Dini-Andreote
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  3. Rodrigo Gouvêa Taketani
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  4. Siu Mui Tsai
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  5. João Lúcio Azevedo
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  6. Itamar Soares de Melo
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  7. Fernando Dini Andreote
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Correspondence to Fernando Dini Andreote.

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Responsible editor: Geraldene Wharton

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Dias, A.C.F., Dini-Andreote, F., Taketani, R.G. et al. Archaeal communities in the sediments of three contrasting mangroves. J Soils Sediments 11, 1466–1476 (2011). https://doi.org/10.1007/s11368-011-0423-7

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  • DOI: https://doi.org/10.1007/s11368-011-0423-7

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