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Effects of light source and inter-species mixed culture on the growth of microalgae and bacteria for nutrient recycling and microalgae harvesting using black odorous water as the medium

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Abstract

To achieve the sustainable and effective removal efficiency of nutrients in black odorous water, light source, inter-species microalgae mixed culture, and the harvesting effect were all explored. The results showed that under a LED light source, the addition of interspecific soluble algal products (SAP) promoted the growth of Haematococcus pluvialis (H. pluvialis) M1, and its maximum specific growth rate was 1.76 times that of H. pluvialis cultivated alone. That was due to the hormesis effect between the two kinds of microalgae, the SAP produced by Scenedesmus could stimulate the growth of H. pluvialis. The algae and bacteria symbiotic system with black odorous water as the medium showed excellent performance to treat nutrients, where the concentration of ammonia nitrogen (NH3-N) and total phosphorus (TP) (0.84, 0.23 mg/L) met the requirements of landscape water. The microbial diversity analysis revealed that the introduction of microalgae changed the dominant species of the bacterial community from Bacteroidota to Proteobacteria. Furthermore, timely microalgae harvesting could prevent water quality from deteriorating and was conducive to microalgae growth and resource recycling. The higher harvest efficiency (98.1%) of H. pluvialis was obtained when an inoculation size of 20% and 0.16 g/L FeCl3 were provided.

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Funding

This work was supported by the Natural Science Foundation of Shandong Province, China (Grant No. ZR2020MB123) & Zibo City Integration Development Project (Grant No. 2019ZBXC392). The authors also wish to express their thanks to Zibo Jinxuan Resources and Environmental Technology Development Co. Ltd. for their sincere help during this work.

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Authors and Affiliations

  1. Department of Resources and Environmental Engineering, Shandong University of Technology, 266 Xincun West Road, Zibo, 255049, China

    Xiaoyan Wang, Mengying Wang, Xiaowei Ma, Huawei Li, Yonghui Zhang, Wanchao Song & Jie Lu

  2. Faculty of Science, University of Melbourne, Parkville, VIC, 3010, Australia

    Shaoxuan Ding

  3. Department of Water Resources and Environmental Engineering, China University of Geosciences, Beijing, 100083, China

    Mengying Wang

  4. School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing, 210044, China

    Xiaowei Ma

  5. College of Chemical Engineering, Shandong University of Technology, Zibo, 255000, China

    Jincheng Ding

Authors
  1. Xiaoyan Wang
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  2. Shaoxuan Ding
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  3. Mengying Wang
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  4. Xiaowei Ma
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  5. Huawei Li
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  6. Yonghui Zhang
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  7. Wanchao Song
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  8. Jincheng Ding
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  9. Jie Lu
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Contributions

All authors contributed to the study conception and design. Conceptualization, software, and writing—original draft preparation were performed by Xiaoyan Wang. Methodology and investigation were performed by Shaoxuan Ding. Validation was performed by Mengying Wang and Xiaowei Ma. Data curation, formal analysis, and investigation were performed by Huawei Li, Yonghui Zhang, and Wanchao Song, respectively. Supervision and funding acquisition were performed by Jincheng Ding. Writing—reviewing and editing, project administration, and funding acquisition were performed by Jie Lu. The first draft of the manuscript was written by Xiaoyan Wang and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jie Lu.

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Wang, X., Ding, S., Wang, M. et al. Effects of light source and inter-species mixed culture on the growth of microalgae and bacteria for nutrient recycling and microalgae harvesting using black odorous water as the medium. Environ Sci Pollut Res 29, 78542–78554 (2022). https://doi.org/10.1007/s11356-022-21293-9

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  • DOI: https://doi.org/10.1007/s11356-022-21293-9

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