Abstract
The nutritional profile of tropical mariculture wastewater in Hainan Island and the possibilities of artificial mariculture wastewater (AWW) bioremediation using microalgae and bacteria screened from the South China Sea were studied. A total of 34 sampling sites from field and water were selected to study the current status of mariaquaculture in Wenchang, an important aquaculture production base in Hainan. The mean value of pH, suspended solids (SS), NH4+-N, PO43−-P, and CODMn of field samples were 7.52, 229.44 mg/L, 1.44 mg/L, 0.88 mg/L, and 9.5 mg/L, respectively. Monocultures of microalgae (Chlorella vulgaris, Platymonas subcordiformis, and Chaetoceros müelleri) and microalgae-bacteria symbiosis (C. vulgaris and Bacillus spp.) were also studied in AWW indoor inoculation experiments. The results of monocultures indicated that C. vulgaris is the optimal strain for removing NH4+-N, NO2−-N, TN, and PO43−-P, with a removal rate of 97.15%, 99.45%, 95.90%, and 96.62% in 72 h, respectively. By comparison, symbiosis of C. vulgaris-Bacillus spp. with an initial concentration of 2.5 × 105 cells/mL microalgae + 5 mL Bacillus spp. (OD600 = 1.8) enhanced the removal of NH4+-N, PO43—P, and CODMn, with a removal rate of 100%, 100%, and 35.69% in 72 h, respectively. Combining microalgae and bacteria communities is probably effective in nutrients removal, enhancing microalgal biomass and biofuel production.
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Acknowledgements
This study was supported by the financial support from the Natural Science Foundation of Hainan Province, China (Grant No. 2019RC043), the National Natural Science Foundation of China (41766003), the start-up funding from Hainan University (kyqd(zr)1719), and the Natural Science Foundation of Hainan Province, China (518QN212). All authors gratefully acknowledge these financial supports.
Funding
This study was supported by the financial support from the Natural Science Foundation of Hainan Province, China (Grant No. 2019RC043), the National Natural Science Foundation of China (41766003), the start-up funding from Hainan University (kyqd(zr)1719), and the Natural Science Foundation of Hainan Province, China (518QN212).
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Ruiyang Ma contributed to the formal analysis, experiment investigation and original draft. Data curation was performed by Linhai Pu and Huiting Jia. Manuscript review and editing were prepared by Ruiyang Ma, Shiyu Xie, Linhai Pu, Huiting Jia, Licheng Peng and Tariq Mehmood. This study was supervised by Licheng Peng and Chengjun Ge. All authors read and approved the final manuscript.
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Highlights
• Mariculture wastewater pollution was detected in Wenchang, Hainan Province, China.
• High removal efficiency of N and P was achieved by Chlorella vulgaris.
• Complete removal of NH4+-N and PO43−-P was found in the symbiosis of C. vulgaris and Bacillus spp.
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Ma, R., Xie, S., Jia, H. et al. Nutritional Profile and Bioremediation of Tropical Marine Aquaculture with an Integrated Microalgae and Bacteria Symbiosis. Water Air Soil Pollut 233, 172 (2022). https://doi.org/10.1007/s11270-022-05615-8
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DOI: https://doi.org/10.1007/s11270-022-05615-8