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The mixed culture of microalgae Chlorella pyrenoidosa and yeast Yarrowia lipolytica for microbial biomass production

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Abstract

Microbial biomass which mostly generated from the microbial processes of bacteria, yeasts, and microalgae is an important resource. Recent concerns in microbial biomass production field, especially microbial lipid production for biofuel, have been focused towards the mixed culture of microalgae and yeast. To more comprehensive understanding of the mixed culture for microbial biomass, mono Chlorella pyrenoidosa, mono Yarrowia lipolytica and the mixed culture were investigated in the present work. Results showed that the mixed culture achieved significantly faster cell propagation of microalga and yeast, smaller individual cell size of yeast and higher relative chlorophyll content of microalga. The mixed culture facilitated the assimilation of carbon and nitrogen and drove the carbon flow to carbohydrate. Besides higher lipid yield (0.77 g/L), higher yields of carbohydrates (1.82 g/L), protein (1.99 g/L) and heating value (114.64 kJ/L) indicated the microbial biomass harvested from the mixed culture have more potential utilization in renewable energy, feedstuff, and chemical industry.

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Acknowledgements

This work was supported by the Sciences and Technology of Guangzhou [Grant no. 201704030084], the National Natural Science Foundation of China [Grant no. 21606230], the Natural Science Foundation for research team of Guangdong Province [Grant no. 2016A030312007], and the National Key Research and Development Program-China [Grant no. 2016YFB0601004]. The authors are grateful to Mohammad Asraful Alam for his kind help in English editing.

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Authors and Affiliations

  1. School of Food Science and Engineering, South China University of Technology, Wushan Rd. 381, Guangzhou, 510641, People’s Republic of China

    Lei Qin, Lu Liu & Dong Wei

  2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Lei Qin & Zhongming Wang

  3. CAS Key Laboratory of Renewable Energy, Guangzhou, 510640, People’s Republic of China

    Lei Qin & Zhongming Wang

  4. Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou, 510640, People’s Republic of China

    Lei Qin & Zhongming Wang

  5. School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637459, Singapore

    Weining Chen

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  1. Lei Qin
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Correspondence to Dong Wei.

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Qin, L., Liu, L., Wang, Z. et al. The mixed culture of microalgae Chlorella pyrenoidosa and yeast Yarrowia lipolytica for microbial biomass production. Bioprocess Biosyst Eng 42, 1409–1419 (2019). https://doi.org/10.1007/s00449-019-02138-1

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  • DOI: https://doi.org/10.1007/s00449-019-02138-1

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