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Composting pig manure and sawdust with urease inhibitor: succession of nitrogen functional genes and bacterial community

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Abstract

Understanding the relationship between nitrogen (N) cycle and N transformation-related functional genes is crucial to reduce N loss during composting process. Urease inhibitor (UI) is widely used to reduce N loss in agriculture. However, the effects of UI on N transformation and related N functional genes during composting have not been well investigated. The goal of this study was to investigate the effects of a urease inhibitor (UI) on N functional genes and bacterial community succession during pig manure composting. Results showed that the addition of UI decreased the ammonium N content during the thermophilic stage and notably increased the total N and nitrite N contents of the final compost. The UI significantly decreased the abundances of amoA, nirS, nirK, and nosZ during the initial composting stage, while the opposite trend was observed at the maturation stage. Bacterial community richness and diversity were increased after the UI amendment, but the relative abundance of the phyla Firmicutes and Proteobacteria significantly decreased compared with control during the thermophilic stage. Redundancy analysis indicated that the evaluated environmental factors and bacterial community showed a cumulative 94.7% contribution to the total variation in N functional genes. In summary, UI addition is a recommended method for N conservation during composting, but the added forms of UI, such as delayed addition, combined with adsorbing materials, or microorganism inoculant, should be further evaluated.

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Funding

Financial support for this investigation was provided by the National Natural Science Foundation of China (41805123), Program for Innovative Research Team in Science and Technology in University of Henan Province (No.20IRTSTHN011), Natural Science Foundation of Henan Province of China (182300410153), and the Key Project of Science and Technology of Henan Province (Nos. 182102210381 and 182102311051).

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Authors and Affiliations

  1. School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, 453007, Henan, People’s Republic of China

    Jishao Jiang, Yang Wang, Fengqi Guo, Xiaofang Zhang, Wei Dong, Xindan Zhang, Xin Zhang, Chunyan Zhang, Ke Cheng, Yunbei Li & Guifen Zhu

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  1. Jishao Jiang
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  2. Yang Wang
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  3. Fengqi Guo
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  4. Xiaofang Zhang
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  5. Wei Dong
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  6. Xindan Zhang
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  7. Xin Zhang
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  10. Yunbei Li
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  11. Guifen Zhu
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Correspondence to Jishao Jiang or Guifen Zhu.

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Jiang, J., Wang, Y., Guo, F. et al. Composting pig manure and sawdust with urease inhibitor: succession of nitrogen functional genes and bacterial community. Environ Sci Pollut Res 27, 36160–36171 (2020). https://doi.org/10.1007/s11356-020-09696-y

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