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Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism

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Abstract

Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4·5H2O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4·5H2O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.

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Acknowledgments

This paper was supported by the Ministry of Education, Science and Technological Development of Republic of Serbia (Grant numbers TR 31080 and OI 172057).

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  1. Danka S. Radić

    Present address: Faculty of Agriculture, University of Belgrade, Nemanjina 6, Belgrade, Zemun, 11080, Serbia

Authors and Affiliations

  1. Faculty of Agriculture, University of Belgrade, Nemanjina 6, Belgrade, Zemun, 11080, Serbia

    Jelena P. Jovičić-Petrović, Vera M. Karličić, Blažo T. Lalević & Vera B. Raičević

  2. Faculty of Mechanical Engineering, University of Belgrade, Kraljice Marije 16, Belgrade, 11000, Serbia

    Vera P. Pavlović

  3. A BIO TECH LAB D.O.O, Vojvode Putnika 87, Sremska Kamenica, 21208, Serbia

    Milana M. Lazović

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  1. Danka S. Radić
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Correspondence to Danka S. Radić.

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Responsible editor: Elena Maestri

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Radić, D.S., Pavlović, V.P., Lazović, M.M. et al. Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. Environ Sci Pollut Res 24, 21885–21893 (2017). https://doi.org/10.1007/s11356-017-9817-4

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  • DOI: https://doi.org/10.1007/s11356-017-9817-4

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