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Characterization of Radiation-Resistant Yeast Isolated from Radiation-Polluted Areas and Its Potential Application in Bioremediation

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Abstract

Twenty-four yeast isolates were obtained from radiation-polluted areas soils samples exposed to 60Co-γ radiation at a dose of 10 kGy and identified as members of the genus Cryptococcus based on morphological and LSU rDNA D1/D2 domain sequence analysis. Further phylogenetic analysis showed that the isolates were clustered into 4 branches. The investigation of these yeast isolates revealed D10 values for γ radiation of 5 (M2), 5 (M38), 6 (M22) and 7 kGy (M5) and a 4 × 10–3% survival rate at a UV dosage of 200 J/m2. Furthermore, the tolerance of M5 towards Zn2+ was as high as 1.700 mg/L. The isolate M5 was selected for further study, including the distribution of heavy metals within the cells by subcellular fractionation, expression of relevant genes involved in heavy metal resistance by qRT-PCR, and the removal rate by flame atomic absorption spectrometry (FAAS). The results revealed that almost 72.9% of the Zn2+ was absorbed by the cells and deposited mainly in the membrane fraction. Compared to normal conditions, all of the genes in M5 strain related to transport and regulation showed greater than 10.4-fold increases of expression levels upon exposure to heavy metal stress. In the presence of culture medium, a total of 86.5% of the initial concentration of Zn2+ was removed by the M5 strain exhibiting high removal efficiency and providing a potential of radiation-resistant platform strain for the bioremediation of heavy metals in polluted environments in the near future.

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ACKNOWLEDGMENTS

The authors wish to thank Professor Y. Shi (Institute of Microbiology, Xinjiang Academy of Agricultural Sciences) for leading us to collect strains, and Professor F. Bai (Institute of Microbiology, Chinese Academy of Sciences) for the help of yeast identification.

Funding

This work was supported by the National Natural Science Foundation of China (31922070), the Natural Science Foundation of Jiangsu Province (BK20180038, BK20171461), the National High Technology Research and Development Program of China (2012AA021705), Tianshan Pine Plan (2017XS26), Basic Scientific R and D Program for Public Welfare Institutes in Xinjiang (KY2019023), and Basic Scientific R & D Program for Public Welfare Institutes in Xinjiang (KY2019019).

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Authors and Affiliations

  1. College of Food Science and Light Industry, Nanjing Tech University, 210009, Nanjing, People’s Republic of China

    T. Liu, X. Liu & L. Jiang

  2. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 210009, Nanjing, People’s Republic of China

    T. Liu, X. Liu & H. Huang

  3. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang Uigur Autonomous Region, People’s Republic of China

    J. Zhu, Q. Tang, W. Wang & Z. Zhang

  4. College of Pharmaceutical Sciences, Nanjing Tech University, 210009, Nanjing, People’s Republic of China

    L. Zhu

  5. Nanjing Beishengrong Energy Technology Co. Ltd., 210009, Nanjing, People’s Republic of China

    Z. Zhang

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  1. T. Liu
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Correspondence to Z. Zhang or L. Jiang.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Liu, T., Liu, X., Zhu, J. et al. Characterization of Radiation-Resistant Yeast Isolated from Radiation-Polluted Areas and Its Potential Application in Bioremediation. Appl Biochem Microbiol 56, 553–562 (2020). https://doi.org/10.1134/S0003683820050117

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