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Fe deficiency induced changes in rice (Oryza sativa L.) thylakoids

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Abstract

Iron deficiency is an important abiotic stress that limits productivity of crops all over the world. We selected a hybrid rice (Oryza sativa L.), LYPJ, which is super high-yield and widely cultured in China, to investigate changes in the components and structure of thylakoid membranes and photosynthetic performance in response to iron deficiency. Our results demonstrated that photosystem I (PSI) is the primary target for iron deficiency, while the changes in photosystem II (PSII) are important for rebuilding a balance in disrupted energy utilization and dissipation caused by differential degradation of photosynthetic components. The result of immunoblot analysis suggested that the core subunit PsaA declined drastically, while PsbA remained relatively stable. Furthermore, several organizational changes of the photosynthetic apparatus were found by BN-PAGE, including a marked decrease in the PSI core complexes, the Cytb 6 /f complex, and the trimeric form of the LHCII antenna, consistent with the observed unstacking grana. The fluorescence induction analysis indicated a descending PSII activity with energy dissipation enhanced markedly. In addition, we proposed that the crippled CO2 assimilation could be compensated by the enhanced of phosphoenolpyruvate carboxylase (PEPC), which is suggested by the decreased ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and photosynthetic efficiency.

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Abbreviations

BN:

Blue native

Chl:

Chlorophyll

DM:

Dodecyl-β-d-maltoside

LHC:

Light harvesting complex

PAGE:

Polyacrylamide gel electrophoresis

PEPC:

Phosphoenolpyruvate carboxylase

PSI:

Photosystem I

PSII:

Photosystem II

SDS:

Sodium dodecyl sulfate

RuBisCO:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 31271621), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and NSFC for Talents Training in Basic Science (J1103507, J1210025).

Authors’ contribution

This work was designed by G. Chen. Data analysis was performed by Y. Wang and M. Wu. Experiments were performed by K. Li and X. Cai. Y. Wang and C. Xu wrote the paper.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China

    Yuwen Wang, Chao Xu, Kang Li, Xiaojie Cai, Min Wu & Guoxiang Chen

  2. Zijin College, Nanjing University of Science and Technology, Nanjing, 210023, China

    Min Wu

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  1. Yuwen Wang
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  2. Chao Xu
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  3. Kang Li
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  4. Xiaojie Cai
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  5. Min Wu
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Correspondence to Guoxiang Chen.

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Wang, Y., Xu, C., Li, K. et al. Fe deficiency induced changes in rice (Oryza sativa L.) thylakoids. Environ Sci Pollut Res 24, 1380–1388 (2017). https://doi.org/10.1007/s11356-016-7900-x

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  • DOI: https://doi.org/10.1007/s11356-016-7900-x

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