Abstract
Jumonji (JMJ) genes play major roles in the modulation of chromatin at certain stages of plant development such as flowering, development of androecium and gynoecium, circadian regulation, and brassinosteroid signalling. However, the information on this gene family in pear (Pyrus bretschneideri) is limited. In this study, 19 PbrJMJ genes were identified and divided into five groups. Their gene structures and conserved domains shared a resemblance within the same groups. 18 PbrJMJ genes were presented on 8 of the 17 chromosomes in the pear. In the expansion of the PbrJMJ genes family, the whole-genome duplication event played a critical role, and the evolution of the PbrJMJ family was mainly affected by purification selection pressure. Gene expression analysis revealed that PbrJMJ genes were distributed in various tissues of P. bretschneideri. PbrJMJ14 and PbrJMJ29 were highly expressed in the pollen. Inhibition of PbrJMJ14 and PbrJMJ29 expression resulted in reduced pollen tube length, suggesting that they play a role in regulating pollen tube growth. In summary, these results provide evolutionary insight into PbrJMJ genes and lay a foundation for further analysis of their functions.
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Acknowledgements
The usage of the microscope was guided by Yuehua Ma.
Data archiving statement
All PbrJMJ-related sequences were available from the Pear Genome Project (peargenome.njau.edu.cn) (Wu et al. 2013). The protein sequences of JMJ members including apple, peach, strawberry, sweet cherry, Rosa chinensis, rice, maize, and soybean were searched and downloaded from the Phytozome (http://phytozome.jgi.doe.gov/) database.
Funding
This work was funded by the National Key Research and Development Program of China (Grant No. 2020YFE0202900), the National Natural Science Foundation of China (32172543), Outstanding Talent for Agricultural Research Project (13210324), the Earmarked Fund for China Agriculture Research System (CARS-28), and the Priority Academic Program Development of Jiangsu Higher Education Institutions. Bioinformatic analysis was supported by the Bioinformatics Center of Nanjing Agricultural University.
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Khan, W., Liu, W., Liu, Z. et al. Genome-wide identification, expression analysis, and functional verification of the JMJ (Jumonji) histone demethylase gene family in pear (Pyrus bretchneideri). Tree Genetics & Genomes 19, 10 (2023). https://doi.org/10.1007/s11295-023-01586-x
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DOI: https://doi.org/10.1007/s11295-023-01586-x