Abstract
Rice straw burning annually (millions of tons) leads to greenhouse gas emissions, and an alternative solution is producing humic acid with high added-value. This study aimed to examine the influence of a microbial consortium and other additives (chicken manure, urea, olive mill waste, zeolite, and biochar) on the composting process of rice straw and the subsequent production of humic acid. Results showed that among the fungal species, Thermoascus aurantiacus exhibited the most prominent impact in expediting maturation and improving compost quality, and Bacillus subtilis was the most abundant bacterial species based on metagenomics analysis. The highest temperature, C/N ratio reduction, and amount of humic acid production (Respectively in lab 61 °C, 54.67%, 298 g kg−1 and in pilot level 65 °C, 72.11%, 310 g kg−1) were related to treatments containing these microorganisms and other additives except urea. Consequently, T. aurantiacus and B. subtilis can be employed on an industrial scale as compost additives to further elevate quality. Functional analysis showed that the bacterial enzymes in the treatments had the highest metabolic activities, including carbohydrate and amino acid metabolism compared to the control. The maximum enzymatic activities were in the thermophilic phase in treatments which were significantly higher than that in the control. The research emphasizes the importance of identifying and incorporating enzymatically active strains that are suitable for temperature conditions, alongside the native strains in decomposing materials. This strategy significantly improves the composting process and yields high-quality humic acid during the thermophilic phase.
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Data Availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Abdel-rahman MA (2016) Establishment of efficient cellulolytic bacterial consortium potential for designed composting of rice straw. Int J Adv Res Biol Sci 3:211–228
Akyol C, Ince O, Ince B (2019) Crop-based composting of lignocellulosic digestates: focus on bacterial and fungal diversity. Bioresour Technol 288:121549. https://doi.org/10.1016/j.biortech.2019.121549
Al-Heetimi OT, Van De Ven CJ, Van Geel PJ et al (2023) Impact of temperature on the performance of compost-based landfill biocovers. J Environ Manag. https://doi.org/10.1016/j.jenvman.2023.118780
Albertsen M, Kars SM, Ziegler AS et al (2015) Back to basics – the influence of DNA extraction and primer choice on phylogenetic analysis of activated sludge communities. PLoS One. https://doi.org/10.1371/journal.pone.0132783
Altieri R, Esposito A, Baruzzi G et al (2014) Corroboration for the successful application of humified olive mill waste compost in soilless cultivation of strawberry. Int Biodeterior Biodegradation 88:118–124
Amalero EG, Ingua GL, Erta GB et al (2003) Review article Methods for studying root colonization by introduced. Agron Afr 23:407–418. https://doi.org/10.1051/agro
Asing J, Wong NS, Lau S (2009) Optimization of extraction method and characterization of humic acid derived from coals and composts. J Trop Agric Fd Sc 37:211–223
Azim K, Ouyahia K, Amellouk A et al (2014) Dynamic composting optimization through C/N ratio variation as a start-up parameter. In: Proceedings of the 4th ISOFAR scientific conference. ‘Building Organic Bridges’, at the Organic World Congress 2014, 13–15 Oct., Istanbul, Turkey 3, pp. 787–790. https://doi.org/10.3220/REP_20_1_2014.
Azim K, Soudi B, Boukhari S et al (2018) Composting parameters and compost quality: a literature review. Org Agric 8:141–158. https://doi.org/10.1007/s13165-017-0180-z
Bahemmat M, Farahbakhsh M, Kianirad M (2016) Humic substances-enhanced electroremediation of heavy metals contaminated soil. J Hazard Mater 312:307–318. https://doi.org/10.1016/j.jhazmat.2016.03.038
Baird RB, Eaton AD, Clesceri LS (2012) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, DC
Chaofan M, Po Kim L, Jiaqi X (2020) Molecular mechanisms underlying lignocellulose degradation and antibiotic resistance genes removal revealed via metagenomics analysis during different agricultural wastes composting. Bioresour Technol 314:1–10. https://doi.org/10.1016/j.biortech.2020.123731
Chen H, Awasthi SK, Liu T et al (2020) Effects of microbial culture and chicken manure biochar on compost maturity and greenhouse gas emissions during chicken manure composting. J Hazard Mater 389:121908. https://doi.org/10.1016/j.jhazmat.2019.121908
Chang HY, Yue YY, Osman SBO et al (2018) Determination of extraction period and extractant ratio for extracting humic acid from rice straw compost. Curr Agric Res J 6:150–156
Cortes-Tolalpa L, Wang Y, Salles JF et al (2020) Comparative genome analysis of the lignocellulose degrading bacteria citrobacter freundii so4 and sphingobacterium multivorum w15. Front Microbiol 11:248. https://doi.org/10.3389/fmicb.2020.00248
Diacono M, Persiani A, Testani E (2019) Recycling agricultural wastes and by-products in organic farming: biofertilizer production, yield performance and carbon footprint analysis. Biotechnol Lett 32:1765–1775
Ding J, Wei D, An Z et al (2020) Succession of the bacterial community structure and functional prediction in two composting systems viewed through metatranscriptomics. Bioresour Technol. https://doi.org/10.1016/j.biortech.2020.123688
Duan CJ, Feng JX (2010) Mining metagenomes for novel cellulase genes. Biotechnol Lett 32:1765–1775. https://doi.org/10.1007/s10529-010-0356-z
Duan H, Ji M, Chen A et al (2021) Evaluating the impact of rice husk on successions of bacterial and fungal communities during cow manure composting. Environ Technol Innov. https://doi.org/10.1016/j.eti.2021.102084
Duan M, Zhang Y, Zhou B (2020) Effects of Bacillus subtilis on carbon components and microbial functional metabolism during cow manure–straw composting. Bioresour Technol 303:122868. https://doi.org/10.1016/j.biortech.2020.122868
Duan Y, Awasthi SK, Chen H et al (2019) Evaluating the impact of bamboo biochar on the fungal community succession during chicken manure composting. Bioresour Technol 272:308–314. https://doi.org/10.1016/j.biortech.2018.10.045
Edgar RC, Haas BJ, Clemente JC et al (2011) UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27:2194–2200. https://doi.org/10.1093/bioinformatics/btr381
Fan H, Liao J, Abass OK, Liu L et al (2018) Effects of compost characteristics on nutrient retention and simultaneous pollutant immobilization and degradation during co-composting process. Bioresour Technol 275:61–69. https://doi.org/10.1016/j.biortech.2018.12.049
Finore I, Feola A, Russo L et al (2023) Thermophilic bacteria and their thermozymes in composting processes: a review. Chem Biol Tech Agri 10:7
Gao Y, Liu S, Wang N et al (2024) Humic acid biosynthesis and bacterial community evolution during aerobic composting of rice straw. Appl Microbiol Biotechnol 108:1–14
García AC, Carlos O, Tavares H et al (2016) Structure-function relationship of vermicompost humic fractions for use in agriculture. J Soils Sediments 18:1365–1375. https://doi.org/10.1007/s11368-016-1521-3
Gavande PV, Basak A, Sen S et al (2021) Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization. Sci Rep 11:1–13. https://doi.org/10.1038/s41598-021-82163-x
Gayathri B, Srinivasamurthy C, Vasanthi B et al (2020) Extraction and charactrisation of humic acid from different organic wastes and its physico-chemical properties. Int J Chem Stud 7:769–775
Gong X, Zou L, Wang L et al (2023) Biochar improves compost humification, maturity and mitigates nitrogen loss during the vermicomposting of cattle manure-maize straw. Environ Manag 325:116432. https://doi.org/10.1016/j.jenvman.2022.116432
Gupta P, Parkhey P (2015) Design of a single chambered microbial electrolytic cell reactor for the production of biohydrogen from rice straw hydrolysate. Biotechnol Lett 37:1213–1219. https://doi.org/10.1007/s10529-015-1780-x
Han Y, Liu W, Chang N et al (2023) Exploration of β-glucosidase-producing microorganisms community structure and key communities driving cellulose degradation during composting of pure corn straw by multi-interaction analysis. Environ Manage 325:116694. https://doi.org/10.1016/j.jenvman.2022.116694
Hettiarachchi L, Jayathilake N, Fernando S et al (2019) Effects of compost particle size, moisture content and binding agents on co-compost pellet properties. Int J Agric Biol Eng 12:184–191
Hidayat B, Sebayang NUW, Akbar AM (2023) Co-composting cow manure, rice straw with marine organic waste: characterization of compost quality. IOP Conference Series: J Earth Environ Sci. https://doi.org/10.1088/1755-1315/1182/1/012029
Hubner T, Herrmann A, Kretzschmar J et al (2019) Suitability of fecal sludge from composting toilets as feedstock for carbonization. Water Sanit Hyg Dev. https://doi.org/10.2166/washdev.2019.047
Jie L, Ya -Ting, C, Zi-Yuan X, et al (2020) Changes in bacterial communities during a pilot-scale composting process of dairy manure. Environ Eng 146(04020095–04020095):13. https://doi.org/10.1061/(ASCE)EE.1943-7870.0001774CITATIONS
Karanja AW, Njeru EM, Maingi JM (2019) Assessment of physicochemical changes during composting rice straw with chicken and donkey manure. IJROWA 8:65–72. https://doi.org/10.1007/s40093-019-0270-x
Kausar H, Ismail MR, Saud HM et al (2013) Use of lignocellulolytic microbial consortium and ph amendment on composting efficacy of rice straw. Compost Sci Util 21:121–133. https://doi.org/10.1080/1065657X.2013.842131
Khemakhem M, Sotiroudis G, Mitsou E et al (2016) Melanin and humic acid-like polymer complex from olive mill waste waters. Part II. Surfactant properties and encapsulation in W/O microemulsions. J Mol Liq 222:480–486. https://doi.org/10.1016/j.molliq.2016.07.065
Kong Z, Wang X, Wang M et al (2020) Bacterial ecosystem functioning in organic matter biodegradation of different composting at the thermophilic phase. Bioresour Technol 317:123990. https://doi.org/10.1016/j.biortech.2020.123990
Lai CMT, Chua HB, Danquah MK et al (2016) Isolation of thermophilic lignin degrading bacteria from oil-palm empty fruit bunch (efb) compost. IOP Conf Ser: Mater Sci Eng. https://doi.org/10.1088/1757-899X/206/1/012016
Lalremruati M, Devi AS (2023) Duration of composting and changes in temperature, pH and C/N ratio during composting: a review. Agric Rev 44:350–356
Li P, Jia L, Chen Q et al (2024) Adaptive evaluation for agricultural sustainability of different fertilizer management options for a green manure-maize rotation system: impacts on crop yield, soil biochemical properties and organic carbon fractions. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2023.168170
Liu H, Huang Y, Wang H et al (2020) Enzymatic activities triggered by the succession of microbiota steered fiber degradation and humification during co-composting of chicken manure and rice husk. J Environ Manage 258:110014. https://doi.org/10.1016/j.jenvman.2019.110014
Liu J, Nauta J, Van Eekert MH et al (2023) Integrated life cycle assessment of biotreatment and agricultural use of domestic organic residues: environmental benefits, trade-offs, and impacts on soil application. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2023.165372
Liu L, Wang S, Guo X et al (2018) Succession and diversity of microorganisms and their association with physicochemical properties during green waste thermophilic composting. Waste Manag 73:101–112. https://doi.org/10.1016/j.wasman.2017.12.026
Liu YX, Qin Y, Chen T et al (2021) A practical guide to amplicon and metagenomic analysis of microbiome data. Protein Cell 12:315–330. https://doi.org/10.1007/s13238-020-00724-8
Malik K, Rani S, Ahlawat S et al (2021) Bioconversion process for compost production from agricultural residue. Int J Chem Stud. 9:827–830
Mang SM, Trotta V, Scopa A et al (2022) Metagenomic analysis of bacterial community structure and dynamics of a digestate and a more stabilized digestate-derived compost from agricultural waste. J Processes 10:2–379. https://doi.org/10.3390/pr10020379
Meena AL, Karwal M, Dutta D et al (2021) Composting: phases and factors responsible for efficient and improved composting. J Agric Food Res 1:85–90
Minz D, Green SJ, Ofek M et al (2010) Compost microbial populations and interactions with plants. Microbes at work: from wastes to resources. Springer
Nagachandrabose S, Sankaranarayanan C, Subramanian KS (2023) The use of humic acid in nematode management. Cambridge Scholars Publishing
Nguyen MK, Lin C, Hoang HG et al (2022) Evaluate the role of biochar during the organic waste composting process: a critical review. Chemosphere. https://doi.org/10.1016/j.chemosphere.2022.134488
Van Nguyen H, Nguyen CD, Tran T et al (2016) Energy efficiency, greenhouse gas emissions, and cost of rice straw collection in the mekong river delta of vietnam. Crop Res. https://doi.org/10.1016/j.fcr.2016.08.024
Paul JW (2001) Composting as a strategy to reduce greenhouse gas emissions. Clim Change 2:3–5
Peng S, Li H, Xu Q et al (2019) Addition of zeolite and superphosphate to windrow composting of chicken manure improves fertilizer efficiency and reduces greenhouse gas emission. Environ Sci Pollut Res 26:36845–36856. https://doi.org/10.1007/s11356-019-06544-6
Pourmazaheri H, Salehi Jouzani G, Karimi E et al (2015) Development of a bioprocess for fast production of enriched biocompost from municipal solid wastes. Int Biodeterior Biodegrad 104:482–489. https://doi.org/10.1016/j.ibiod.2015.08.002
Puyuelo B, Gea T, Sanchez A (2014) GHG emissions during the high-rate production of compost using standard and advanced aeration strategies. Chemosphere 109:64–70. https://doi.org/10.1016/j.chemosphere.2014.02.060
Qu F, Cheng H, Han Z et al (2023) Identification of driving factors of lignocellulose degrading enzyme genes in different microbial communities during rice straw composting. Bioresour Technol 381:129109. https://doi.org/10.1016/j.biortech.2023.129109
Rashid GMM, Charles RT, Yangqingxue L et al (2015) Identification of manganese superoxide dismutase from Sphingobacterium sp. T2 as a novel bacterial enzyme for lignin oxidation. ACS Chem Biol 10:2286–2294. https://doi.org/10.1021/acschembio.5b00298
Rathour RK, Devi M, Dahiya P et al (2023) Recent trends, opportunities and challenges in sustainable management of rice straw waste biomass for green biorefinery. J Energy 16:1429. https://doi.org/10.3390/en16031429
Ravindra K, Singh T, Mor S (2019) Emissions of air pollutants from primary crop residue burning in India and their mitigation strategies for cleaner emissions. J Clean Prod 208:261–273. https://doi.org/10.1016/j.jclepro.2018.10.031
Reddy AP, Simmons CW, Patrik D et al (2013) Discovery of microorganisms and enzymes involved in high-solids decomposition of rice straw using metagenomic analyses. PLoS ONE 8:10. https://doi.org/10.1371/journal.pone.0077985
Ren G, Xu X, Qu J et al (2016) Evaluation of microbial population dynamics in the co-composting of cow manure and rice straw using high throughput sequencing analysis. World J Microbiol Biotechnol 32:1–11. https://doi.org/10.1007/s11274-016-2059-7
Ren L, Yan B, Awasthi MK et al (2021) Accelerated humification and alteration of microbial communities by distillers’ grains addition during rice straw composting. Bioresour Technol 342:125937. https://doi.org/10.1016/j.biortech.2021.125937
Roulia M (2024) Sustainable utilization of humic substances and organic waste in green agriculture. J Agric 14:1–115. https://doi.org/10.3390/agriculture14010115
Sadik S, Mazouz H, Bouaichi A et al (2013) Biological control of bacterial onion diseases using a bacterium, Pantoea agglomerans. J Sci Res 4:103–111
Schloss PDL, Westcott S, Ryabin T et al (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 23:7537–7541
Shams M, Nabipour I, Dobaradaran S et al (2014) An environmental friendly and cheap adsorbent (municipal solid waste compost ash) with high efficiency in removal of phosphorus from aqueous solution. Fresenius Environ Bull 3:722–726
Stancampiano LM, Verrillo M, Cangemi S et al (2023) the molecular composition of humic substances extracted from green composts and their potential for soil remediation. Environ Chem Lett 12:1797. https://doi.org/10.1007/s10311-023-01619-w
Su Q, Wu Y, Wang S et al (2023) The reverse function of lignin-degrading enzymes: the polymerization ability to promote the formation of humic substances in domesticated composting. Bioresour Technol 380:129059. https://doi.org/10.1016/j.biortech.2023.129059
Sun R, Wang X, Alhaj Hamoud Y et al (2023) Dynamic variation of bacterial community assemblage and functional profiles during rice straw degradation. Front Microbiol 14:1173442. https://doi.org/10.3389/fmicb.2023.1173442
Tiwari J, Ramanathan A, Bauddh K et al (2023) Humic substances: structure, function and benefits for agroecosystems—a review. Pedosphere 33:237–249. https://doi.org/10.1016/j.pedsph.2022.07.008
Vergara S, Silver W (2019) Greenhouse gas emissions from windrow composting of organic wastes: patterns and emissions factors. Environ Res Lett 14:12. https://doi.org/10.1088/1748-9326/ab5262
Wang C, Dong D, Wang H et al (2016a) Metagenomic analysis of microbial consortia enriched from compost: New insights into the role of Actinobacteria in lignocellulose decomposition. Biotechnol Biofuels 9:1–17. https://doi.org/10.1186/s13068-016-0440-2
Wang R, Li D, Deng F (2024) Production of artificial humic acid from rice straw for fertilizer production and soil improvement. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2023.167548
Wang WK, Liang CM (2021) Enhancing the compost maturation of swine manure and rice straw by applying bioaugmentation. J Sci Rep 11:6103. https://doi.org/10.1038/s41598-021-85615-6
Wang W, Yan L, Cui Z et al (2011) Characterization of a microbial consortium capable of degrading lignocellulose. Bioresour Technol. https://doi.org/10.1016/j.biortech.2011.07.065
Wang Y, Fan C, Hu H et al (2016b) Genetic modification of plant cell walls to enhance biomass yield and biofuel production in bioenergy crops. Biotechnol Adv 34:997–1017. https://doi.org/10.1016/j.biotechadv.2016.06.001
Wang Y, Huang Z, Sheng L et al (2023) Effect of modified humic acid residue on the adsorption and passivation of Hg2+/Pb2+ in solution and soil. Mol Liq 27:12–8932. https://doi.org/10.1016/j.molliq.2023.121581
Wang Z, Cao M, Cai W et al (2017) The effect of humic acid and fulvic acid on adsorption-desorption behavior of copper and zinc in the yellow soil. AIP Conf Proc. https://doi.org/10.1063/1.4977299
Wang Z, Wu D, Lin Y et al (2021) Role of temperature in sludge composting and hyperthermophilic systems: a review. Bioenergy Res 15:962–976. https://doi.org/10.1007/s12155-021-10281-5
Wei Q, Wang X, Feng Y et al (2024) Dynamics of physicochemical properties, microbial composition, and antibiotic and antibiotic resistance genes during chicken manure composting with strain T4. J Soils Sediments. https://doi.org/10.1007/s11368-024-03755-4
Wei T, Qian S, Dabing X et al (2012) Succession of bacterial communities during composting process as detected by 16S rRNA clone libraries analysis. Int Biodeterior Biodegradation 78:58–66. https://doi.org/10.1016/j.ibiod.2012.12.008
Willis AD (2019) Rarefaction, alpha diversity and statistics. Front Microbiol 10:1–5. https://doi.org/10.3389/fmicb.2019.02407
Xie G, Kong X, Kang J et al (2021) Community-level dormancy potential regulates bacterial beta-diversity succession during the co-composting of manure and crop residues. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2021.145506
Yaldız G, Camlıca M, Ozen F et al (2019) Effect of poultry manure on yield and nutrient composition of sweet basil (Ocimum basilicum L.). Commun Soil Sci Plant Anal 50:838–852. https://doi.org/10.1080/00103624.2019.1589488
Yang R, Li Z, Huang B et al (2018) Effects of Fe(III)-fulvic acid on Cu removal via adsorption versus coprecipitation. Chemosphere 197:291–298. https://doi.org/10.1016/j.chemosphere.2018.01.042
Yu H, Li P, Bo G et al (2024) Studies on the humic acid structure and microbial nutrient restriction mechanism during organic-inorganic co-composting. J Environ Manag. https://doi.org/10.1016/j.jenvman.2024.120186
Zainudin MHM, Hassan MA, Tokura M et al (2013) Indigenous cellulolytic and hemicellulolytic bacteria enhanced rapid co- composting of lignocellulose oil palm empty fruit bunch with palm oil mill effluent anaerobic sludge. Bioresour Technol 147:632–635. https://doi.org/10.1016/j.biortech.2013.08.061
Zainudin MH, Mustapha NA, Maeda T et al (2020) Biochar enhanced the nitrifying and denitrifying bacterial communities during the composting of poultry manure and rice straw. J Waste Manag 106:240–249. https://doi.org/10.1016/j.wasman.2020.03.029
Zainudin MHM, Singam JT, Sazili AQ et al (2022) Indigenous cellulolytic aerobic and facultative anaerobic bacterial community enhanced the composting of rice straw and chicken manure with biochar addition. J Sci Rep 12:5930. https://doi.org/10.1038/s41598-022-09789-3
Zhan Y, Chang Y, Tao Y et al (2023) Insight into the dynamic microbial community and core bacteria in composting from different sources by advanced bioinformatics methods. Environ Sci Pollut Res 30:8956–8966. https://doi.org/10.1007/s11356-022-20388-7
Zhang Y, Liu G, Gao S et al (2023) Effect of humic acid on phytoremediation of heavy metal contaminated sediment. JHM Adv 31:4051–4060. https://doi.org/10.1016/j.scitotenv.2023.165385
Zhao L, Zhao M, Gao W et al (2024) Different Bacillus sp. play different roles on humic acid during lignocellulosic biomass composting. J Clean Prod. https://doi.org/10.1016/j.jclepro.2023.139901
Yi Z, Zhao Y, Zhang Z et al (2017) Effect of thermo-tolerant actinomycetes inoculation on cellulose degradation and the formation of humic substances during composting. J Waste Manag 68:64–73. https://doi.org/10.1016/j.wasman.2017.06.022
Zhong XZ, Li XX, Zeng Y et al (2020) Dynamic change of bacterial community during dairy manure composting process revealed by high-throughput sequencing and advanced bioinformatics tools. Bioresour Technol 306:123091. https://doi.org/10.1016/j.biortech.2020.123091
Zhou G, Xu X, Qiu X et al (2018) Biochar influences the succession of microbial communities and the metabolic functions during rice straw composting with pig manure. Bioresour Technol 272:10–18. https://doi.org/10.1016/j.biortech.2018.09.135
Zhu N, Zhu Y, Kan Z et al (2021) Effects of two-stage microbial inoculation on organic carbon turnover and fungal community succession during co-composting of cattle manure and rice straw. Bioresour Technol 341:125842. https://doi.org/10.1016/j.biortech.2021.125842
Zhu X, Liu J, Li L et al (2023) Prospects for humic acids treatment and recovery in wastewater: a review. Chemosphere. https://doi.org/10.1016/j.chemosphere.2022.137193
Zhu Y, McBride MJ (2017) The unusual cellulose utilization system of the aerobic soil bacterium Cytophaga hutchinsonii. J Appl Microbiol 101:7113–7127. https://doi.org/10.1007/s00253-017-8467-2
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The research leading to these results received funding from the Agricultural Biotechnology Research Institute of Iran (ABRII) and Agricultural Research, Education and Extension (AREEO) under Grant Agreement No 2–05-05–017-960740.
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Reza Sharafi and Gholamreza Salehi Jouzani conceived and designed research. Reza Sharafi and Ebrahim Karimi conducted experiments. Hosein Ghanavati and Mojegan Kowsari contributed new reagents or analytical tools. Reza Sharafi analyzed data. Reza Sharafi, Gholamreza Salehi Jouzani and Ebrahim Karimi wrote the manuscript. All authors read and approved the manuscript.
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Sharafi, R., Salehi Jouzani, G., Karimi, E. et al. Integrating bioprocess and metagenomics studies to enhance humic acid production from rice straw. World J Microbiol Biotechnol 40, 173 (2024). https://doi.org/10.1007/s11274-024-03959-3
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DOI: https://doi.org/10.1007/s11274-024-03959-3