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Dynamics of physicochemical properties, microbial composition, and antibiotic and antibiotic resistance genes during chicken manure composting with strain T4

  • Soils, Sec 5 • Soil and Landscape Ecology • Research Article
  • Published:
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Abstract

Purpose

Co-composting of chicken manure and edible mushroom residue inoculated with oxytetracycline-degrading strain T4 was conducted to reduce the risk of residual oxytetracycline and tetracycline resistance genes (TRGs) to the ecological environment.

Materials and methods

Three treatments (CK: without bacteria agent T4 inoculated; AM: 1% bacteria agent T4 inoculated in mesophilic phase; AC: 1% bacteria agent T4 inoculated in cooling phase) were set to investigate dynamics of physicochemical properties, microbial composition, and antibiotic and antibiotic resistance genes.

Results and discussion

The bacteria agent T4 inoculated in the mesophilic phase was conductive to the reservation of nutrients and maturity of chicken manure and improved oxytetracycline degradation (t1/2 was 2.77 days, and the total degradation rate was 80.78%). Bacterial community dynamic analysis showed that strain T4 reduced microbial richness and diversity in composting. In addition, the bacteria agent T4 could reduce the relative abundance of encoding efflux pump (EFP) TRGs significantly, and inoculated in the mesophilic phase was better than the cooling phase while having little effect on ribosomal protection protein (RPP) TRGs and enzymatic inactivation (EI) TRG.

Conclusions

Inoculation of strain T4 in the initial stage of composting could be a promising technology to solve the problem of oxytetracycline residue and its resistance genes in chicken manure. It was also found that the abundance of TRGs was affected by bacterial composition and dynamic, which was affected by the physicochemical properties of composting and fates of oxytetracycline, and vice versa.

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Data availability

Data will be made available on request.

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Funding

This research was supported by the National Natural Science Foundation of China (52370141) and the earmarked fund for CARS (China Agriculture Research System, No. CARS-29-zp-10).

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Authors and Affiliations

  1. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Qihang Wei

  2. State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Qihang Wei, Xiaoxing Wang, Yao Feng & Zhaojun Li

  3. Organic Recycling Research Institute (Suzhou) of China Agricultural University, Suzhou, 215168, China

    Qihang Wei

  4. School of Environmental Science and Engineering, Changzhou University, Changzhou, 213164, China

    Yanfang Ren & Junyu He

Authors
  1. Qihang Wei
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  2. Xiaoxing Wang
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  3. Yao Feng
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  4. Yanfang Ren
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  5. Junyu He
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Contributions

Qihang Wei: conceptualization, methodology, investigation, validation, data curation, visualization, and writing—original draft. Xiaoxing Wang: data curation, visualization, and formal analysis. Yao Feng: methodology, investigation, and validation. Yanfang Ren: formal analysis and writing—review and editing. Junyu He: formal analysis and writing—review and editing. Zhaojun Li: conceptualization, funding acquisition, project administration, resources, and supervision.

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Correspondence to Zhaojun Li.

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Wei, Q., Wang, X., Feng, Y. et al. Dynamics of physicochemical properties, microbial composition, and antibiotic and antibiotic resistance genes during chicken manure composting with strain T4. J Soils Sediments 24, 1750–1763 (2024). https://doi.org/10.1007/s11368-024-03755-4

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