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Characterization and evaluation of the cholesterol-lowering ability of Lactiplantibacillus plantarum HJ-S2 isolated from the intestine of Mesoplodon densirostris

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Abstract

In this study, ten strains of lactic acid bacteria were isolated from the intestine of Blainville's beaked whale (Mesoplodon densirostris),and their cholesterol-lowering activities in vitro and in vivo were investigated. The among these strains, HJ-S2 strain, which identified as Lactiplantibacillus plantarum, showed a high in vitro cholesterol-lowering rate (48.82%). Strain HJ-S2 was resistant to acid and bile salts, with a gastrointestinal survival rate of more than 80%, but was sensitive to antibiotics. Strain HJ-S2 was found to be able to adhere to HT-29 cells in an adhesion test. The number of cell adhesion was 132.52. In addition, we also performed the cholesterol-lowering activities in vivo using high-fat diet feed mouse models. Our results indicated that HJ-S2 reduced total cholesterol (TC), total glycerol (TG), and low-density lipoprotein cholesterol (LDLC) levels while increasing the high-density lipoprotein cholesterol (HDLC) level. It also alleviated the lipid accumulation in high-fat diet feed mouse liver and pancreas. Hence, HJ-S2 demonstrated appropriate cholesterol-lowering ability and has the potential to be used as a probiotic in functional foods.

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Data Availability

The data that support the findings of this study are available from the corresponding author, [Xu Tang], upon reasonable request.

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Funding

This study was supported by the Natural Science Foundation of Fujian Province of China (2020J01103) and Xiamen Southern Oceanographic Center (21CZP003HJ13).

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  1. Jingliang Wan and Peng Wu contributed equally to this work.

Authors and Affiliations

  1. Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China

    Jingliang Wan, Peng Wu, Jiaqi Huang, Shixin Huang & Xu Tang

  2. Fujian Huisheng Biological Technology Co., Ltd, Zhangzhou, China

    Qinmiao Huang

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  1. Jingliang Wan
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Contributions

JW and PW performed the experiments equally; JW wrote the original draft preparation; PW and XT revised the main manuscript text; JH and SH prepared Figs. 1–6 and Table 1–5. JH, SH and QH provided technically support for the study; and XT supervised and managed the study. All authors read and approved the final manuscript.

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Correspondence to Xu Tang.

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Wan, J., Wu, P., Huang, J. et al. Characterization and evaluation of the cholesterol-lowering ability of Lactiplantibacillus plantarum HJ-S2 isolated from the intestine of Mesoplodon densirostris. World J Microbiol Biotechnol 39, 199 (2023). https://doi.org/10.1007/s11274-023-03637-w

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