Abstract
Blakeslea trispora, a heterothallic Zygomycota with two mating types (termed “plus” and “minus”), is an ideal source of lycopene and β-carotene. The lycopene and β-carotene yields when the two type strains are used for fermentation separately are lower than those when they are joint together. To enhance the yield of lycopene and β-carotene in B. trispora, protoplast fusion technology was carried out between ATCC 14,271 (+) and ATCC 14,272 (−). After protoplast preparation, protoplast fusion, fusion sorting, fusion regeneration, and high-throughput screening, two fusions (Fu-1and Fu-2) with high lycopene and β-carotene yields were obtained. The lycopene yields of Fu-1 and Fu-2 were increased to 0.60 mg/gDW and 0.90 mg/gDW, which were respectively 3.62- and 5.44-fold those of 14,271 and 1.76- and 2.64-fold those of 14,272. The β-carotene yields of Fu-1 and Fu-2 were increased to 22.07 mg/gDW and 36.93 mg/gDW, which were respectively 1.72- and 2.89-fold those of 14,271 and 1.23- and 2.06-fold those of 14,272. In this study, the protoplast fusion technique was successfully used in Blakeslea trispora, providing new ideas for improving lycopene and β-carotene production.
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Acknowledgements
This work was supported by the grant of the Supporting Fund for Teachers’ research of Jining Medical University (No. JY2017JS009), the Doctoral Setup Foundation of Jining Medical University (No. 2017JYQD09), the NSFC cultivation project of Jining Medical University (No. JYP2019KJ21), and the Genomics Innovation Team of Systemic Lupus Erythematosus of Jining Medical University. We are grateful to Jining Medical University for the financial support to carry out this study.
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Wang, Y., Wang, Y., Chen, X. et al. Protoplast fusion between Blakeslea trispora 14,271 (+) and 14,272 (−) enhanced the yield of lycopene and β-carotene. World J Microbiol Biotechnol 37, 58 (2021). https://doi.org/10.1007/s11274-021-03023-4
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DOI: https://doi.org/10.1007/s11274-021-03023-4